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Chemical characterisation of Piper amalago (Piperaceae) essential oil by comprehensive two-dimensional gas chromatography coupled with rapid-scanning quadrupole mass spectrometry (GC×GC/qMS) and their antilithiasic activity and acute toxicity.
Dos Santos, Anaí L; Novaes, Antônio da Silva; Polidoro, Allan Dos S; de Barros, Márcio Eduardo; Mota, Jonas S; Lima, Daiane B M; Krause, Laiza C; Cardoso, Cláudia A L; Jacques, Rosângela A; Caramão, Elina B.
Afiliação
  • Dos Santos AL; Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • Novaes ADS; Hospital da Universidade Federal da Grande Dourados, Dourados, MS, Brazil.
  • Polidoro ADS; Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • de Barros ME; Hospital da Universidade Federal da Grande Dourados, Dourados, MS, Brazil.
  • Mota JS; Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados, Dourados, MS, Brazil.
  • Lima DBM; Centro de Estudos em Recursos Naturais, Universidade Estadual do Mato Grosso do Sul, Dourados, MS, Brazil.
  • Krause LC; Departamento de Biotecnologia Industrial, Universidade Tiradentes, Aracaju, SE, Brazil.
  • Cardoso CAL; Departamento de Biotecnologia Industrial, Universidade Tiradentes, Aracaju, SE, Brazil.
  • Jacques RA; Centro de Estudos em Recursos Naturais, Universidade Estadual do Mato Grosso do Sul, Dourados, MS, Brazil.
  • Caramão EB; Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Phytochem Anal ; 29(5): 432-445, 2018 Sep.
Article em En | MEDLINE | ID: mdl-29479742
INTRODUCTION: Piper amalago has a distribution from Mexico to Brazil; their aerial parts have been used in folk medicine to treat diuretic and kidney diseases. OBJECTIVE: The purpose of this study was to obtain a deeper understanding of the chemical composition of essential oils (EOs) extracted from both the leaves and stems of P. amalago, compare them, and evaluate their antilithiasic activity and acute toxicity. METHODOLOGY: Extraction was performed by hydrodistillation, whereas chemical characterisation by two-dimensional gas chromatography coupled with rapid-scanning quadrupole mass spectrometry (GC×GC/qMS). The antilithiasic activity was evaluated by the effect of the EOs on calcium oxalate crystallisation in vitro. The turbidity index and the number of crystals formed were determined and used as an estimative of the activity. In the acute toxicity assay, the effects of a single oral dose of the EOs in Wistar rats were determined. General behaviour, adverse effects, and mortality were determined. RESULTS: A total of 322 compounds were identified in the EOs. The sesquiterpenes displayed the highest contribution in leaves EOs among which included bicyclogermacrene and δ-cadinene. Sesquiterpenes and oxygenated sesquiterpenes displayed the highest contribution in EOs from stems, among which included bicyclogermacrene and α-cadinol. The EOs demonstrated an excellent action on the crystals growth inhibition, and the oral dose tested did not induce significant changes in the parameters for acute toxicity. CONCLUSION: The oils have a high chemical complexity, and there are differences between their compositions, which could explain the observed differences in antilithiasic activity. The findings support the use of this plant in folk medicine to treat kidney diseases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óleos Voláteis / Cálculos Renais / Piper / Cromatografia Gasosa-Espectrometria de Massas Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Phytochem Anal Assunto da revista: BOTANICA / QUIMICA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óleos Voláteis / Cálculos Renais / Piper / Cromatografia Gasosa-Espectrometria de Massas Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Phytochem Anal Assunto da revista: BOTANICA / QUIMICA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido