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Antibodies to human neutrophil antigen HNA-3b implicated in cases of neonatal alloimmune neutropenia.
Lopes, Larissa Barbosa; Abbas, Samira Ali; Moritz, Elyse; Martins, Juliana Oliveira; Chiba, Akemi Kuroda; Langhi, Dante Mário; Bordin, José O.
Afiliação
  • Lopes LB; Disciplina de Hematologia e Hemoterapia da Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
  • Abbas SA; Disciplina de Hematologia e Hemoterapia da Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
  • Moritz E; Disciplina de Hematologia e Hemoterapia da Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
  • Martins JO; Disciplina de Hematologia e Hemoterapia da Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
  • Chiba AK; Disciplina de Hematologia e Hemoterapia da Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
  • Langhi DM; Disciplina de Hematologia e Hemoterapia da Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
  • Bordin JO; Disciplina de Hematologia e Hemoterapia da Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
Transfusion ; 58(5): 1264-1270, 2018 05.
Article em En | MEDLINE | ID: mdl-29451309
BACKGROUND: Neonatal alloimmune neutropenia results from maternal alloimmunization to human neutrophil antigens. The alloantibodies involved in neonatal alloimmune neutropenia are against human neutrophil antigens HNA-1a, HNA-1b, HNA-1c, HNA-1d, HNA-2, HNA-3a, HNA-4a, HNA-4b, and HNA-5a; however, to date, antibodies specific to HNA-3b have not been reported. STUDY DESIGN AND METHODS: Blood samples from 10,000 unselected neonates were analyzed, resulting in the selection of 88 neutropenic newborns (neutrophil count <1.5 × 109 /L) from 83 mothers (three pairs of twins and one triplet). HNA-3 genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism to identify the cases of maternal-fetal HNA-3 incompatibility. Serologic studies for detecting maternal HNA-3 alloantibodies were performed with the granulocyte agglutination test, the white blood cell immunofluorescence test, and a LABScreen Multi-HNA Kit. RESULTS: Genotyping studies identified 13 of 88 (14.8%) instances of maternal-fetal HNA-3 incompatibility, with all mothers typed as HNA-3a/a and neonates typed as HNA-3a/b. Serologic studies revealed that five of 13 (38.5%) mothers carried anti-HNA-3b plus human leukocyte antigen antibodies and that three of 13 (23.1%) mothers had anti-HNA-3b without human leukocyte antigen antibodies. CONCLUSION: Here, we report the first three cases of neonatal alloimmune neutropenia associated with HNA-3b antibodies resulting in a neonatal alloimmune neutropenia incidence of one in 3333 live births.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças do Recém-Nascido / Isoanticorpos / Isoantígenos / Neutropenia Tipo de estudo: Etiology_studies Limite: Humans / Infant / Newborn Idioma: En Revista: Transfusion Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças do Recém-Nascido / Isoanticorpos / Isoantígenos / Neutropenia Tipo de estudo: Etiology_studies Limite: Humans / Infant / Newborn Idioma: En Revista: Transfusion Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos