Your browser doesn't support javascript.
loading
Effect of prior application with and without post-injury treatment with low-level laser on the modulation of key proteins in the muscle repair process.
De Lima Rodrigues, Danielle; Alves, Agnelo Neves; Guimarães, Beatriz Ribeiro; de Alcântara Araujo Amorim, Weslley Wallace; Bussadori, Sandra Kalil; Fernandes, Kristianne Porta Santos; Mesquita-Ferrari, Raquel Agnelli.
Afiliação
  • De Lima Rodrigues D; Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho - UNINOVE, São Paulo, SP, Brazil.
  • Alves AN; Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho - UNINOVE, São Paulo, SP, Brazil.
  • Guimarães BR; Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho - UNINOVE, São Paulo, SP, Brazil.
  • de Alcântara Araujo Amorim WW; Universidade Nove de Julho - UNINOVE, São Paulo, SP, Brazil.
  • Bussadori SK; Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho - UNINOVE, São Paulo, SP, Brazil.
  • Fernandes KPS; Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho - UNINOVE, São Paulo, SP, Brazil.
  • Mesquita-Ferrari RA; Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho - UNINOVE, São Paulo, SP, Brazil.
Lasers Med Sci ; 33(6): 1207-1213, 2018 Aug.
Article em En | MEDLINE | ID: mdl-29450762
The aim of the present study was to evaluate the effects of LLLT prior to muscle injury with and without post-injury irradiation on the expression of isoforms of myosin heavy chain (MyHC), calcineurin (CaN), and myostatin during the repair process. Wistar rats were divided into five groups: control (n = 7); injury (n = 21); LLLT + injury (n = 21); injury + LLLT (n = 21), and LLLT + injury + LLLT (n = 21). Cryoinjury was performed on the tibialis anterior (TA) muscle. The injured groups were euthanized at 3, 7, and 14 days after injury. LLLT was performed using an infrared laser (780 nm) with the following parameters: 10 J/cm2, 40 mW, 10 s per point, 8 points, and 3.2 J of total energy. At the end of each period, the TA muscle was removed for the analysis of MyHC, CaN, and myostatin gene expression using real-time PCR. The data were tested statistically by Kruskal-Wallis with Dunn's post hoc test (p < 0.05). The results demonstrated that prior irradiation reduced the mRNA expression of all proteins at 3 days. Post irradiation reduced the mRNA expression of MyHC-1, MyHC-2a, MyHC-2b, and CaN at 7 days. Prior irradiation combined with post-injury irradiation reduced the mRNA expression of MyHC-2x and CaN at 14 days and increased the mRNA expression of myostatin in the same period. In conclusion, different protocols of photobiomodulation can modulate the expression of the different isoforms of MyHC, CaN, and myostatin during the repair process. It is noteworthy that the combination of the prior and post-injury irradiation was the protocol that most promoted changes in the final phase of the repair process.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regeneração / Cicatrização / Músculo Esquelético / Terapia com Luz de Baixa Intensidade / Proteínas Musculares Limite: Animals Idioma: En Revista: Lasers Med Sci Assunto da revista: BIOTECNOLOGIA / RADIOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regeneração / Cicatrização / Músculo Esquelético / Terapia com Luz de Baixa Intensidade / Proteínas Musculares Limite: Animals Idioma: En Revista: Lasers Med Sci Assunto da revista: BIOTECNOLOGIA / RADIOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido