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Short-term treatment with metformin reduces hepatic lipid accumulation but induces liver inflammation in obese mice.
de Souza Teixeira, Alexandre Abilio; Souza, Camila O; Biondo, Luana A; Sanches Silveira, Loreana; Lima, Edson A; Batatinha, Helena A; Araujo, Adriane Pereira; Alves, Michele Joana; Hirabara, Sandro Massao; Curi, Rui; Neto, José Cesar Rosa.
Afiliação
  • de Souza Teixeira AA; Immunometabolism Research Group, Department of Cell Biology and Development, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil. alexandreast@gmail.com.
  • Souza CO; Immunometabolism Research Group, Department of Cell Biology and Development, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Biondo LA; Immunometabolism Research Group, Department of Cell Biology and Development, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Sanches Silveira L; Exercise and Immunometabolism Research Group, Department of Physical Education, UNESP, Presidente Prudente, Brazil.
  • Lima EA; Immunometabolism Research Group, Department of Cell Biology and Development, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Batatinha HA; Immunometabolism Research Group, Department of Cell Biology and Development, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Araujo AP; Immunometabolism Research Group, Department of Cell Biology and Development, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Alves MJ; Immunometabolism Research Group, Department of Cell Biology and Development, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Hirabara SM; Cruzeiro do Sul University, São Paulo, SP, Brazil.
  • Curi R; Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Neto JCR; Cruzeiro do Sul University, São Paulo, SP, Brazil.
Inflammopharmacology ; 26(4): 1103-1115, 2018 Aug.
Article em En | MEDLINE | ID: mdl-29450671
The study aimed to evaluate the metabolic and inflammatory effects of short-term treatments (10 days) with metformin (MET) on the NAFLD caused by a high-fat diet (HFD) in C57BL/6 mice. After the treatment, histological liver slices were obtained, hepatocytes and macrophages were extracted and cultured with phosphate buffered saline, LPS (2.5 µg/mL) and MET (1 µM) for 24 h. Cytokine levels were determined by ELISA. NAFLD caused by the HFD was partially reduced by MET. The lipid accumulation induced by the HFD was not associated with liver inflammation; however, MET seemed to promote pro-inflammatory effects in liver, since it increased hepatic concentration of IL-1ß, TNF-α, IL-6, MCP-1 and IFN-γ. Similarly, MET increased the concentration of IL-1ß, IL-6 in hepatocyte cultures. However, in macrophages culture, MET lowered levels of IL-1ß, IL-6 and TNF-α stimulated by LPS. Overall, MET reduced liver NAFLD but promoted hepatocyte increase in pro-inflammatory cytokines, thus, leading to liver inflammation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citocinas / Hepatopatia Gordurosa não Alcoólica / Inflamação / Metformina Limite: Animals Idioma: En Revista: Inflammopharmacology Assunto da revista: FARMACOLOGIA / TERAPIA POR MEDICAMENTOS Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citocinas / Hepatopatia Gordurosa não Alcoólica / Inflamação / Metformina Limite: Animals Idioma: En Revista: Inflammopharmacology Assunto da revista: FARMACOLOGIA / TERAPIA POR MEDICAMENTOS Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça