Your browser doesn't support javascript.
loading
Identification and evaluation of Galleria mellonella peptides with antileishmanial activity.
Patiño-Márquez, Isabel A; Patiño-González, Edwin; Hernández-Villa, Laura; Ortíz-Reyes, Blanca; Manrique-Moreno, Marcela.
Afiliação
  • Patiño-Márquez IA; Faculty of Exact and Natural Sciences, University of Antioquia, A.A. 1226, Medellin, Colombia.
  • Patiño-González E; Faculty of Exact and Natural Sciences, University of Antioquia, A.A. 1226, Medellin, Colombia.
  • Hernández-Villa L; Faculty of Exact and Natural Sciences, University of Antioquia, A.A. 1226, Medellin, Colombia.
  • Ortíz-Reyes B; Faculty of Medicine, University of Antioquia, A.A. 1226, Medellin, Colombia.
  • Manrique-Moreno M; Faculty of Exact and Natural Sciences, University of Antioquia, A.A. 1226, Medellin, Colombia. Electronic address: marcela.manrique@udea.edu.co.
Anal Biochem ; 546: 35-42, 2018 04 01.
Article em En | MEDLINE | ID: mdl-29409865
Leishmaniasis is a neglected disease, World Health Organization (WHO) declared it as high priority worldwide. Colombia is one of the 98 countries in which the disease caused more than 17.000 cases per year. There is a need to explore novel therapies to reduce the side effects of the current treatments. For this reason, this study was aimed to evaluate Galleria mellonella hemolymph for potential peptides with anti-parasitic activity. Larvae were challenged with Leishmania (V) panamensis promastigotes and hemolymph was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), reversed-phase chromatography (RP-HPLC), two-dimensional gel electrophoresis and liquid chromatography-mass spectroscopy (LC/MS). The immunological response of Galleria mellonella was followed by SDS-PAGE, immunized hemolymph was fractionated by RP-HPLC where fractions 5 and 11 showed the highest antileishmanial activity. From these fractions 15 spots were isolated by 2D gel electrophoresis and evaluated by LC/MS to identify the peptides present in the spots. After the analysis Moricin-B, Moricin-C4, Cecropin-D and Anionic Peptide 2 were identified due to the immune challenge with Leishmania promastigotes. Anionic peptide 2 and Cecropin-D were synthesized and evaluated for antileishmanial activity. The results showed that Anionic peptide 2 presented more anti-parasitic activity. This study showed for the first time the anti-parasitic potential of peptides derived from hemolymph of Galleria mellonella.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos Catiônicos Antimicrobianos / Leishmania / Lepidópteros / Antiprotozoários Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Anal Biochem Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos Catiônicos Antimicrobianos / Leishmania / Lepidópteros / Antiprotozoários Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Anal Biochem Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Estados Unidos