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Diversity and enzymatic potentialities of Bacillus sp. strains isolated from a polluted freshwater ecosystem in Cuba.
Larrea-Murrell, Jeny Adina; Rojas-Badia, Marcia Maria; García-Soto, Ivette; Romeu-Alvarez, Beatriz; Bacchetti, Tristano; Gillis, Annika; Boltes-Espinola, Ana Karina; Heydrich-Perez, Mayra; Lugo-Moya, Daysi; Mahillon, Jacques.
Afiliação
  • Larrea-Murrell JA; Laboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty, Havana University, Havana, Cuba.
  • Rojas-Badia MM; Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
  • García-Soto I; Laboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty, Havana University, Havana, Cuba. marcia@fbio.uh.cu.
  • Romeu-Alvarez B; Laboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty, Havana University, Havana, Cuba.
  • Bacchetti T; Laboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty, Havana University, Havana, Cuba.
  • Gillis A; Instituto Madrileño de Estudios Avanzados Agua (IMDEA Agua). C/Punto Net 4, Parque Científico Tecnológico de la Universidad de Alcalá, 28805, Alcalá de Henares, Madrid, Spain.
  • Boltes-Espinola AK; Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
  • Heydrich-Perez M; Instituto Madrileño de Estudios Avanzados Agua (IMDEA Agua). C/Punto Net 4, Parque Científico Tecnológico de la Universidad de Alcalá, 28805, Alcalá de Henares, Madrid, Spain.
  • Lugo-Moya D; Laboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty, Havana University, Havana, Cuba.
  • Mahillon J; Laboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty, Havana University, Havana, Cuba.
World J Microbiol Biotechnol ; 34(2): 28, 2018 Jan 19.
Article em En | MEDLINE | ID: mdl-29350293
Genotypic and phenotypic characterization of Bacillus spp. from polluted freshwater has been poorly addressed. The objective of this research was to determine the diversity and enzymatic potentialities of Bacillus spp. strains isolated from the Almendares River. Bacilli strains from a polluted river were characterized by considering the production of extracellular enzymes using API ZYM. 14 strains were selected and identified using 16S rRNA, gyrB and aroE genes. Genotypic diversity of the Bacillus spp. strains was evaluated using pulsed field gel electrophoresis. Furthermore, the presence of genetic determinants of potential virulence toxins of the Bacillus cereus group and proteinaceous crystal inclusions of Bacillus thuringiensis was determined. 10 strains were identified as B. thuringiensis, two as Bacillus megaterium, one as Bacillus pumilus and one as Bacillus subtilis. Most strains produced proteases, amylases, phosphatases, esterases, aminopeptidases and glucanases, which reflect the abundance of biopolymeric matter in Almendares River. Comparison of the typing results revealed a spatio-temporal distribution among B. thuringiensis strains along the river. The results of the present study highlight the genotypic and phenotypic diversity of Bacillus spp. strains from a polluted river, which contributes to the knowledge of genetic diversity of Bacilli from tropical polluted freshwater ecosystems.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus / Microbiologia da Água / Ecossistema / Biodiversidade / Água Doce País/Região como assunto: Caribe / Cuba Idioma: En Revista: World J Microbiol Biotechnol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Cuba País de publicação: Alemanha
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus / Microbiologia da Água / Ecossistema / Biodiversidade / Água Doce País/Região como assunto: Caribe / Cuba Idioma: En Revista: World J Microbiol Biotechnol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Cuba País de publicação: Alemanha