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Quantitative evaluation of collagen and elastic fibers after intense pulsed light treatment of mouse skin.
Faucz, Luciana L; Will, Sonia E; Rodrigues, Consuelo J; Hesse, Henrique; Moraes, Angelina C; Maria, Durvanei A.
Afiliação
  • Faucz LL; Laboratory of Biochemistry and Biophysics of Butantan Institute, University of Sao Paulo, Butantã, Sao Paulo, Brazil.
  • Will SE; Dermatology Service, Faculty of Medicine, Hospital Universitário Evangelico de Curitiba; Faculdade Evangélica do Paraná, Curitiba, Brazil.
  • Rodrigues CJ; Laboratory of Biochemistry and Biophysics of Butantan Institute, University of Sao Paulo, Butantã, Sao Paulo, Brazil.
  • Hesse H; Faculty of Medicine, Department of Orthopedics and Traumatology, University of Sao Paulo, Sao Paulo, Brazil.
  • Moraes AC; University of Santa Cruz do Sul, Santa Cruz do Sul, Rio Grande do Sul, Brazil.
  • Maria DA; Laboratory of Biochemistry and Biophysics of Butantan Institute, University of Sao Paulo, Butantã, Sao Paulo, Brazil.
Lasers Surg Med ; 2018 Jan 16.
Article em En | MEDLINE | ID: mdl-29336034
BACKGROUND AND OBJECTIVE: The aging of human skin includes intrinsic aging and photo-aging, which are characterized by a decrease in collagen and the deposition of abnormal elastic fibers. Intense pulsed light (IPL) sources are widely used in medicine to treat various cosmetic problems, including photo-damaged skin. Few studies have examined the microscopic changes produced by IPL. The objective of this study was to quantitatively evaluate the effects of IPL on collagen and elastic fibers in mice. MATERIALS AND METHODS: Forty female BALB/c mice were divided into four subgroups. Group 1 was the control group (n = 10), and groups 2, 3, and 4 were treatment groups (n = 10 in each group). Group 2 received one treatment, group 3 received two treatments, and group 4 received three treatments every 2 weeks. Skin tissue was obtained from irradiated areas 24 hours after the last treatment in each mouse. Collagen fibers were identified using the picrosirius red method. Elastic fibers were marked by Weigert-oxone stain. All samples were analyzed and quantified by a light microscope using analyzer system images. RESULTS: Group 4, which received three IPL treatments, showed significant quantitative increases in both collagen fibers (P < 0.05) and elastic fibers (P < 0.01). Collagen fibers demonstrated a better parallel distribution in relation to the epidermis. CONCLUSION: IPL treatment significantly increased the number of collagen and elastic fibers within the dermis and improved the parallel distribution of collagen fibers in relation to the epidermis. These results were evident after three IPL treatments. Lasers Surg. Med. © 2018 Wiley Periodicals, Inc.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Lasers Surg Med Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Lasers Surg Med Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos