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Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food.
Pacheco Coello, Ricardo; Pestana Justo, Jorge; Factos Mendoza, Andrés; Santos Ordoñez, Efrén.
Afiliação
  • Pacheco Coello R; ESPOL Polytechnic University, Escuela Superior Politécnica del Litoral, ESPOL, Centro de Investigaciones Biotecnológicas del Ecuador, Campus Gustavo Galindo, Km. 30.5 vía Perimetral, P.O. Box 09-01-5863, Guayaquil, Ecuador.
  • Pestana Justo J; Agencia Nacional de Regulación, Control y Vigilancia Sanitaria, ARCSA, Ciudadela Samanes, Av. Francisco de Orellana y Av. Paseo del Parque, Bloque 5, Guayaquil, Ecuador.
  • Factos Mendoza A; Biosafety Unit, National Biodiversity Direction, Ministry of Environment, Madrid y Andalucía, Quito, Ecuador.
  • Santos Ordoñez E; ESPOL Polytechnic University, Escuela Superior Politécnica del Litoral, ESPOL, Centro de Investigaciones Biotecnológicas del Ecuador, Campus Gustavo Galindo, Km. 30.5 vía Perimetral, P.O. Box 09-01-5863, Guayaquil, Ecuador. gsantos@espol.edu.ec.
BMC Res Notes ; 10(1): 758, 2017 Dec 20.
Article em En | MEDLINE | ID: mdl-29262852
OBJECTIVES: In Ecuador, food products need to be labeled if exceeded 0.9% of transgenic content in whole products. For the detection of genetically modified organisms (GMOs), three DNA extraction methods were tested in 35 food products commercialized in Ecuador. Samples with positive amplification of endogenous genes were screened for the presence of the Cauliflower mosaic virus 35S-promoter (P35S) and the nopaline synthase-terminator (Tnos). TaqMan™ probes were used for determination of transgenic content of the GTS 40-3-2 and MON810 events through quantitative PCR (qPCR). RESULTS: Twenty-six processed food samples were positive for the P35S alone and eight samples for the Tnos and P35S. Absolute qPCR results indicated that eleven samples were positive for GTS 40-3-2 specific event and two for MON810 specific event. A total of nine samples for events GTS 40-3-2 and MON810 exceeded the umbral allowed of transgenic content in the whole food product with the specific events. Different food products may require different DNA extraction protocols for GMO detection through PCR. Among the three methods tested, the DNeasy mericon food kit DNA extraction method obtained higher proportion of amplified endogenous genes through PCR. Finally, event-specific GMOs were detected in food products in Ecuador.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plantas Geneticamente Modificadas / DNA de Plantas / Produtos Agrícolas / Análise de Perigos e Pontos Críticos de Controle Tipo de estudo: Diagnostic_studies País/Região como assunto: America do sul / Ecuador Idioma: En Revista: BMC Res Notes Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Equador País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plantas Geneticamente Modificadas / DNA de Plantas / Produtos Agrícolas / Análise de Perigos e Pontos Críticos de Controle Tipo de estudo: Diagnostic_studies País/Região como assunto: America do sul / Ecuador Idioma: En Revista: BMC Res Notes Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Equador País de publicação: Reino Unido