Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food.
BMC Res Notes
; 10(1): 758, 2017 Dec 20.
Article
em En
| MEDLINE
| ID: mdl-29262852
OBJECTIVES: In Ecuador, food products need to be labeled if exceeded 0.9% of transgenic content in whole products. For the detection of genetically modified organisms (GMOs), three DNA extraction methods were tested in 35 food products commercialized in Ecuador. Samples with positive amplification of endogenous genes were screened for the presence of the Cauliflower mosaic virus 35S-promoter (P35S) and the nopaline synthase-terminator (Tnos). TaqMan™ probes were used for determination of transgenic content of the GTS 40-3-2 and MON810 events through quantitative PCR (qPCR). RESULTS: Twenty-six processed food samples were positive for the P35S alone and eight samples for the Tnos and P35S. Absolute qPCR results indicated that eleven samples were positive for GTS 40-3-2 specific event and two for MON810 specific event. A total of nine samples for events GTS 40-3-2 and MON810 exceeded the umbral allowed of transgenic content in the whole food product with the specific events. Different food products may require different DNA extraction protocols for GMO detection through PCR. Among the three methods tested, the DNeasy mericon food kit DNA extraction method obtained higher proportion of amplified endogenous genes through PCR. Finally, event-specific GMOs were detected in food products in Ecuador.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas Geneticamente Modificadas
/
DNA de Plantas
/
Produtos Agrícolas
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Análise de Perigos e Pontos Críticos de Controle
Tipo de estudo:
Diagnostic_studies
País/Região como assunto:
America do sul
/
Ecuador
Idioma:
En
Revista:
BMC Res Notes
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Equador
País de publicação:
Reino Unido