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Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells.
Montiel-Dávalos, Angélica; Silva Sánchez, Guadalupe Jazmin; Huerta-García, Elizabeth; Rueda-Romero, Cristhiam; Soca Chafre, Giovanny; Mitre-Aguilar, Irma B; Alfaro-Moreno, Ernesto; Pedraza-Chaverri, José; López-Marure, Rebeca.
Afiliação
  • Montiel-Dávalos A; Subdirección de Investigación Básica, Instituto Nacional de Cancerología, Ciudad de México, México.
  • Silva Sánchez GJ; Subdirección de Investigación Básica, Instituto Nacional de Cancerología, Ciudad de México, México.
  • Huerta-García E; Departamento de Fisiología (Biología Celular), Instituto Nacional de Cardiología "Ignacio Chávez", Ciudad de México, México.
  • Rueda-Romero C; Subdirección de Investigación Básica, Instituto Nacional de Cancerología, Ciudad de México, México.
  • Soca Chafre G; Subdirección de Investigación Básica, Instituto Nacional de Cancerología, Ciudad de México, México.
  • Mitre-Aguilar IB; Unidad de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Ciudad de México, México.
  • Alfaro-Moreno E; Swetox, Karolinska Institutet, Unit of Toxicology Sciences, Forskargatan, SE Södertälje, Sweden.
  • Pedraza-Chaverri J; Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Ciudad de México, México.
  • López-Marure R; Departamento de Fisiología (Biología Celular), Instituto Nacional de Cardiología "Ignacio Chávez", Ciudad de México, México.
PLoS One ; 12(12): e0188169, 2017.
Article em En | MEDLINE | ID: mdl-29244817
Curcumin has protective effects against toxic agents and shows preventive properties for various diseases. Particulate material with an aerodynamic diameter of ≤10 µm (PM10) and titanium dioxide nanoparticles (TiO2-NPs) induce endothelial dysfunction and activation. We explored whether curcumin is able to attenuate different events related to endothelial activation. This includes adhesion, expression of adhesion molecules and oxidative stress induced by PM10 and TiO2-NPs. Human umbilical vein endothelial cells (HUVEC) were treated with 1, 10 and 100 µM curcumin for 1 h and then exposed to PM10 at 3 µg/cm2 or TiO2-NPs at 10 µg/cm2. Cell adhesion was evaluated by co-culture with U937 human myelomonocytic cells. Adhesion molecules expression was measured by flow cytometry after 3 or 24 h of exposure. Oxidative stress was determined by 2,7-dichlorodihydrofluorescein (H2DCF) oxidation. PM10 and TiO2-NPs induced the adhesion of U937 cells and the expression of E- and P-selectins, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and platelet-endothelial cell adhesion molecule-1 (PECAM-1). The expression of E- and P-selectins matched the adhesion of monocytes to HUVEC after 3 h. In HUVEC treated with 1 or 10 µM curcumin, the expression of adhesion molecules and monocytes adhesion was significantly diminished. Curcumin also partially reduced the H2DCF oxidation induced by PM10 and TiO2-NPs. Our results suggest an anti-inflammatory and antioxidant role by curcumin attenuating the activation caused on endothelial cells by exposure to particles. Therefore, curcumin could be useful in the treatment of diseases where an inflammatory process and endothelial activation are involved.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Curcumina / Material Particulado / Nanopartículas / Células Endoteliais da Veia Umbilical Humana / Antioxidantes Limite: Humans País/Região como assunto: Mexico Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Curcumina / Material Particulado / Nanopartículas / Células Endoteliais da Veia Umbilical Humana / Antioxidantes Limite: Humans País/Região como assunto: Mexico Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de publicação: Estados Unidos