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The Wnt/ß-catenin pathway is deregulated in cemento-ossifying fibromas.
Pereira, Thaís Dos Santos Fontes; Diniz, Marina Gonçalves; França, Josiane Alves; Moreira, Rennan Garcias; Menezes, Grazielle Helena Ferreira de; Sousa, Sílvia Ferreira de; Castro, Wagner Henriques de; Gomes, Carolina Cavaliéri; Gomez, Ricardo Santiago.
Afiliação
  • Pereira TDSF; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Diniz MG; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • França JA; Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Moreira RG; Genomics Multi-user Laboratory, Biological Sciences Institute, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Menezes GHF; Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Sousa SF; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Castro WH; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Gomes CC; Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Gomez RS; Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Minas Gerais, Brazil. Electronic address: Rsgomez@ufmg.br.
Oral Surg Oral Med Oral Pathol Oral Radiol ; 125(2): 172-178, 2018 02.
Article em En | MEDLINE | ID: mdl-29239811
OBJECTIVE: The molecular pathogenesis of cemento ossifying fibroma (COF) is unclear. The purpose of this study was to investigate mutations in 50 oncogenes and tumor suppressor genes, including APC and CTNNB1, in which mutations in COF have been previously reported. In addition, we assessed the transcriptional levels of the Wnt/ß-catenin pathway genes in COF. STUDY DESIGN: We used a quantitative polymerase chain reaction array to evaluate the transcriptional levels of 44 Wnt/ß-catenin pathway genes in 6 COF samples, in comparison with 6 samples of healthy jaws. By using next-generation sequencing (NGS) in 7 COF samples, we investigated approximately 2800 mutations in 50 genes. RESULTS: The expression assay revealed 12 differentially expressed Wnt/ß-catenin pathway genes in COF, including the upregulation of CTNNB1, TCF7, NKD1, and WNT5 A, and downregulation of CTNNBIP1, FRZB, FZD6, RHOU, SFRP4, WNT10 A, WNT3 A, and WNT4, suggesting activation of the Wnt/ß-catenin signaling pathway. NGS revealed 5 single nucleotide variants: TP53 (rs1042522), PIK3 CA (rs2230461), MET (rs33917957), KIT (rs3822214), and APC (rs33974176), but none of them was pathogenic. CONCLUSIONS: Although NGS detected no oncogenic mutation, deregulation of key Wnt/ß-catenin signaling pathway genes appears to be relevant to the molecular pathogenesis of COF.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Mandibulares / Fibroma Ossificante / Via de Sinalização Wnt Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Oral Surg Oral Med Oral Pathol Oral Radiol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Mandibulares / Fibroma Ossificante / Via de Sinalização Wnt Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Oral Surg Oral Med Oral Pathol Oral Radiol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos