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Calcium chloride-enriched calcium aluminate cement promotes in vitro osteogenesis.
Castro-Raucci, L M S; Teixeira, L N; Barbosa, A F S; Fernandes, R R; Raucci-Neto, W; Jacobovitz, M; Oliveira, I R; de Oliveira, P T.
Afiliação
  • Castro-Raucci LMS; School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
  • Teixeira LN; School of Dentistry, University of Ribeirão Preto, Ribeirão Preto, Brazil.
  • Barbosa AFS; School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
  • Fernandes RR; Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, Brazil.
  • Raucci-Neto W; School of Dentistry, University of Ribeirão Preto, Ribeirão Preto, Brazil.
  • Jacobovitz M; School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
  • Oliveira IR; School of Dentistry, University of Ribeirão Preto, Ribeirão Preto, Brazil.
  • de Oliveira PT; School of Dentistry, Centro Universitário Paulista Central, São Carlos, Brazil.
Int Endod J ; 51(6): 674-683, 2018 Jun.
Article em En | MEDLINE | ID: mdl-29226342
AIM: To evaluate the effects of 2.8% or 10% calcium chloride (CaCl2 ) in calcium aluminate cement (CAC) with either bismuth oxide (Bi2 O3 ) or zinc oxide (ZnO) as radiopacifiers on the progression of osteogenic cell cultures. METHODOLOGY: Rat calvaria-derived cells were grown on Thermanox® coverslips for 24 h and exposed to samples of (i) CACb: with 2.8% CaCl2 and 25% Bi2 O3 ; (ii) CACb+: with 10% CaCl2 and 25% Bi2 O3 ; (iii) CACz: with 2.8% CaCl2 and 25% ZnO; or (iv) CACz+: with 10% CaCl2 and 25% ZnO, placed on inserts. Nonexposed cultures served as the control. Calcium and phosphorus contents in culture media were quantified. The effects of the cements on cell apoptosis, cell viability and acquisition of the osteogenic cell phenotype were evaluated. Data were compared by Kruskal-Wallis test (α = 5%). RESULTS: CACb+ promoted the highest levels of calcium in the culture media; CACz+, the lowest levels of phosphorus (P < 0.05). CACz+ and CACb increased cell apoptosis (P < 0.05). CACb reduced cell viability (P < 0.05) and the expression of the osteoblastic phenotype. CACz+ and CACb+ promoted greater cell differentiation and matrix mineralization compared to CACz and CACb (P < 0.05). CONCLUSION: For CAC with the lower CaCl2 content, the use of Bi2 O3 was detrimental for osteoblastic cell survival and differentiation compared to ZnO, while CAC with the higher CaCl2 content supported the acquisition of the osteogenic cell phenotype in vitro regardless of the radiopacifier used. Thus, CAC with 10% CaCl2 would potentially promote bone repair in the context of endodontic therapies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteogênese / Cloreto de Cálcio / Compostos de Cálcio / Compostos de Alumínio / Cimentos Dentários Limite: Animals Idioma: En Revista: Int Endod J Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteogênese / Cloreto de Cálcio / Compostos de Cálcio / Compostos de Alumínio / Cimentos Dentários Limite: Animals Idioma: En Revista: Int Endod J Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido