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Isolation and molecular characterization of Leishmania infantum in urine from patients with visceral leishmaniasis in Brazil.
da Costa Lima, Manoel Sebastião; Hartkopf, Andressa Cristina Lopes; de Souza Tsujisaki, Rosianne A; Oshiro, Elisa Teruya; Shapiro, Julie Teresa; de Fatima Cepa Matos, Maria; Cavalheiros Dorval, Maria Elizabeth.
Afiliação
  • da Costa Lima MS; Laboratory of Immunopathology and Molecular Biology, FIOCRUZ - Instituto Aggeu Magalhães, Recife, PE, Brazil. Electronic address: manoel.lima@cpqam.fiocruz.br.
  • Hartkopf ACL; Laboratory of Molecular Biology and Cell Culture, Universidade Federal de Mato Grosso do Sul,Av. Senador Filinto Müller, s/n., Campo Grande, MS, Brazil.
  • de Souza Tsujisaki RA; Laboratory of Molecular Biology and Cell Culture, Universidade Federal de Mato Grosso do Sul,Av. Senador Filinto Müller, s/n., Campo Grande, MS, Brazil.
  • Oshiro ET; Center for Biological and Health Sciences, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
  • Shapiro JT; School of Natural Resources and the Environment and Department of Wildlife Ecology and Conservation, University of Florida, Gainesville, FL, 32601, USA.
  • de Fatima Cepa Matos M; Laboratory of Molecular Biology and Cell Culture, Universidade Federal de Mato Grosso do Sul,Av. Senador Filinto Müller, s/n., Campo Grande, MS, Brazil.
  • Cavalheiros Dorval ME; Graduate Program in Infectious and Parasitic Diseases, Universidade Federal de Mato Grosso do Sul, Faculdade de Medicina, Unidade IX, Caixa postal 549, Campo Grande, MS 79070-900, Brazil.
Acta Trop ; 178: 248-251, 2018 Feb.
Article em En | MEDLINE | ID: mdl-29221850
Leishmania infantum is a protozoan that causes visceral leishmaniasis, a potentially deadly neglected tropical disease. The gold standard for diagnosis has traditionally been detection of amastigotes in bone marrow or spleen aspirates, but this is an invasive procedure that carries the risk of serious complications. Newer PCR techniques are opening new avenues and tissues for testing. Therefore, we tested if amastigotes and DNA from L. infantum could be detected in patient urine. We detected L. infantum DNA in six out of 30 urine samples from patients with visceral leishmaniasis and the promastigotes were isolated in culture from the urine of one patient. These results suggest the feasibility of using urine samples to diagnose visceral leishmaniasis, especially in acute cases or renal infection, providing a valuable tool for doctors and clinicians to use for screening and diagnosis of leishmaniasis in patients.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leishmania infantum / Leishmaniose Visceral Limite: Animals / Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Acta Trop Ano de publicação: 2018 Tipo de documento: Article País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leishmania infantum / Leishmaniose Visceral Limite: Animals / Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Acta Trop Ano de publicação: 2018 Tipo de documento: Article País de publicação: Holanda