Bacterial nanocellulose-IKVAV hydrogel matrix modulates melanoma tumor cell adhesion and proliferation and induces vasculogenic mimicry in vitro.
J Biomed Mater Res B Appl Biomater
; 106(8): 2741-2749, 2018 11.
Article
em En
| MEDLINE
| ID: mdl-29206331
Vasculogenic mimicry process has generated great interest over the past decade. So far, however, there have been only a few matrices available that allow us to study that process in vitro. Here, we have developed an innovative hydrogel platform with defined composition that mimics the structural architecture and biological functions of the extracellular matrix for vasculogenic mimicry of human melanoma cells (SK-MEL-28). We chemically immobilized IKVAV peptide on bacterial nanocellulose (BNC) fibers. BNC-IKVAV hydrogel was found to improve the adhesion and proliferation of SK-MEL-28 cells on the top and bottom surfaces. Particularly, the bottom surface of BNC-IKVAV induced SK-MEL-28 cells to organize themselves as well-established networks related to the vasculogenic mimicry process. Finally, our results showed that not only BNC-IKVAV but also BNC hydrogels can potentially be used as a three-dimensional platform that allows the screening of antitumor drugs. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2741-2749, 2018.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
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Bactérias
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Adesão Celular
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Celulose
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Laminina
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Hidrogéis
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Proliferação de Células
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Nanoestruturas
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Melanoma
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Neovascularização Patológica
Limite:
Animals
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Humans
Idioma:
En
Revista:
J Biomed Mater Res B Appl Biomater
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Estados Unidos