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A new therapeutic proposal for inoperable osteosarcoma: Photodynamic therapy.
de Miguel, Guilherme Chohfi; Abrantes, Ana Margarida; Laranjo, Mafalda; Grizotto, Ana Yoshie Kitagawa; Camporeze, Bruno; Pereira, José Aires; Brites, Gonçalo; Serra, Arménio; Pineiro, Marta; Rocha-Gonsalves, António; Botelho, Maria Filomena; Priolli, Denise Gonçalves.
Afiliação
  • de Miguel GC; Laboratory of Multidisciplinary Research, Postgraduate Program in Health Sciences, São Francisco University, Bragança Paulista, SP, Brazil.
  • Abrantes AM; Institute of Biophysics, CIMAGO, CNC.IBILI, Faculty of Medicine of University of Coimbra, Portugal.
  • Laranjo M; Institute of Biophysics, CIMAGO, CNC.IBILI, Faculty of Medicine of University of Coimbra, Portugal.
  • Grizotto AYK; Laboratory of Multidisciplinary Research, Postgraduate Program in Health Sciences, São Francisco University, Bragança Paulista, SP, Brazil.
  • Camporeze B; Laboratory of Multidisciplinary Research, Postgraduate Program in Health Sciences, São Francisco University, Bragança Paulista, SP, Brazil.
  • Pereira JA; Laboratory of Multidisciplinary Research, Postgraduate Program in Health Sciences, São Francisco University, Bragança Paulista, SP, Brazil.
  • Brites G; Institute of Biophysics, Faculty of Medicine of University of Coimbra, Portugal.
  • Serra A; Chemical Engineering Department, Faculty of Sciences and Technology of University of Coimbra, Portugal.
  • Pineiro M; Department of Chemistry, Faculty of Sciences and Technology of University of Coimbra, Portugal.
  • Rocha-Gonsalves A; Department of Chemistry, Faculty of Sciences and Technology of University of Coimbra, Portugal.
  • Botelho MF; Institute of Biophysics, CIMAGO, CNC.IBILI, Faculty of Medicine of University of Coimbra, Portugal.
  • Priolli DG; Laboratory of Multidisciplinary Research, Postgraduate Program in Health Sciences, São Francisco University, Bragança Paulista, SP, Brazil. Electronic address: denise.priolli@usf.edu.br.
Photodiagnosis Photodyn Ther ; 21: 79-85, 2018 Mar.
Article em En | MEDLINE | ID: mdl-29175213
BACKGROUND: Osteosarcoma, a malignant tumor characterized by bone or osteoid formation, is the second most common primary bone neoplasm. Clinical symptoms include local and surrounding pain, unrelieved by rest or anesthesia. Osteosarcoma has a poor chemotherapeutic response with prognosis dependent on complete tumor excision. Therefore, for inoperable osteosarcoma new therapeutic strategies are needed. The present study aimed to develop murine models of cranial and vertebral osteosarcoma that facilitate simple clinical monitoring and real-time imaging to evaluate the outcome of photodynamic therapy based on a previously developed photosensitizer. METHODS: Balb/c nude mice were divided into two groups: the cranial and vertebral osteosarcoma groups. Each group was further subdivided into the photodynamic therapy-treated and untreated groups. Images were obtained by scintigraphy with 99mTc-MIBI and radiography. Tumor growth, necrotic area, osteoid matrix area, and inflammatory infiltration were analyzed. RESULTS: Cranial and vertebral tumors could be macroscopically observed and measured. Radiographic and scintigraphic images showed tumor cells present at the inoculation sites. After photodynamic therapy, scintigraphy showed lower tumoral radiopharmaceutical uptake, which correlated histologically with increased necrosis. Osteoid matrix volume increased, and tumor size decreased in all photodynamic therapy-treated animals. CONCLUSION: Cranial and vertebral osteosarcoma models in athymic mice are feasible and facilitate in vivo monitoring for the development of new therapies. Photodynamic therapy is a potential antitumoral treatment for surgically inoperable osteosarcoma.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Porfirinas / Neoplasias Ósseas / Osteossarcoma / Fármacos Fotossensibilizantes Limite: Animals Idioma: En Revista: Photodiagnosis Photodyn Ther Assunto da revista: DIAGNOSTICO POR IMAGEM / TERAPEUTICA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Porfirinas / Neoplasias Ósseas / Osteossarcoma / Fármacos Fotossensibilizantes Limite: Animals Idioma: En Revista: Photodiagnosis Photodyn Ther Assunto da revista: DIAGNOSTICO POR IMAGEM / TERAPEUTICA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda