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A DNA segment encoding the anticodon stem/loop of tRNA determines the specific recombination of integrative-conjugative elements in Acidithiobacillus species.
Castillo, Andrés; Tello, Mario; Ringwald, Kenneth; Acuña, Lillian G; Quatrini, Raquel; Orellana, Omar.
Afiliação
  • Castillo A; a Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina , Universidad de Chile , Santiago , Región Metropolitana , Chile.
  • Tello M; b Centro de Biotecnología Acuícola, Departamento de Biología, Facultad de Química y Biología , Universidad de Santiago de Chile , Santiago , Chile.
  • Ringwald K; c Carl R. Woese Institute for Genomic Biology, Department of Microbiology , University of Illinois , Urbana-Champaign , Illinois , United States.
  • Acuña LG; d Fundación Ciencia y Vida. Ave. Zañartu 1482 - Ñuñoa, Santiago , Región Metropolitana , Chile.
  • Quatrini R; d Fundación Ciencia y Vida. Ave. Zañartu 1482 - Ñuñoa, Santiago , Región Metropolitana , Chile.
  • Orellana O; a Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina , Universidad de Chile , Santiago , Región Metropolitana , Chile.
RNA Biol ; 15(4-5): 492-499, 2018.
Article em En | MEDLINE | ID: mdl-29168417
Horizontal gene transfer is crucial for the adaptation of microorganisms to environmental cues. The acidophilic, bioleaching bacterium Acidithiobacillus ferrooxidans encodes an integrative-conjugative genetic element (ICEAfe1) inserted in the gene encoding a tRNAAla. This genetic element is actively excised from the chromosome upon induction of DNA damage. A similar genetic element (ICEAcaTY.2) is also found in an equivalent position in the genome of Acidithiobacillus caldus. The local genomic context of both mobile genetic elements is highly syntenous and the cognate integrases are well conserved. By means of site directed mutagenesis, target site deletions and in vivo integrations assays in the heterologous model Escherichia coli, we assessed the target sequence requirements for site-specific recombination to be catalyzed by these integrases. We determined that each enzyme recognizes a specific small DNA segment encoding the anticodon stem/loop of the tRNA as target site and that specific positions in these regions are well conserved in the target attB sites of orthologous integrases. Also, we demonstrate that the local genetic context of the target sequence is not relevant for the integration to take place. These findings shed new light on the mechanism of site-specific integration of integrative-conjugative elements in members of Acidithiobacillus genus.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Bacteriano / RNA de Transferência de Alanina / Elementos de DNA Transponíveis / Transferência Genética Horizontal / Acidithiobacillus Tipo de estudo: Prognostic_studies Idioma: En Revista: RNA Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Bacteriano / RNA de Transferência de Alanina / Elementos de DNA Transponíveis / Transferência Genética Horizontal / Acidithiobacillus Tipo de estudo: Prognostic_studies Idioma: En Revista: RNA Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos