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Predicting CK2 beta-dependent substrates using linear patterns.
Núñez de Villavicencio-Díaz, Teresa; Mazola, Yuliet; Perera Negrín, Yasser; Cruz García, Yiliam; Guirola Cruz, Osmany; Perea Rodríguez, Silvio E.
Afiliação
  • Núñez de Villavicencio-Díaz T; Bioinformatics Group, Department of Systems Biology, Biomedical Research Area, Center for Genetic Engineering and Biotechnology, Playa, La Habana, Cuba.
  • Mazola Y; Bioinformatics Group, Department of Systems Biology, Biomedical Research Area, Center for Genetic Engineering and Biotechnology, Playa, La Habana, Cuba.
  • Perera Negrín Y; Molecular Oncology Group, Cancer Department, Biomedical Research Area, Center for Genetic Engineering and Biotechnology, Playa, La Habana, Cuba.
  • Cruz García Y; Department of Preclinical Studies, National Institute of Oncology and Radiobiology of Cuba, Plaza de la Revolución, La Habana, Cuba.
  • Guirola Cruz O; Bioinformatics Group, Department of Systems Biology, Biomedical Research Area, Center for Genetic Engineering and Biotechnology, Playa, La Habana, Cuba.
  • Perea Rodríguez SE; Molecular Oncology Group, Cancer Department, Biomedical Research Area, Center for Genetic Engineering and Biotechnology, Playa, La Habana, Cuba.
Biochem Biophys Rep ; 4: 20-27, 2015 Dec.
Article em En | MEDLINE | ID: mdl-29124183
CK2 is a constitutively active Ser/Thr protein kinase deregulated in cancer and other pathologies, responsible for about the 20% of the human phosphoproteome. The holoenzyme is a complex composed of two catalytic (α or α´) and two regulatory (ß) subunits, with individual subunits also coexisting in the cell. In the holoenzyme, CK2ß is a substrate-dependent modulator of kinase activity. Therefore, a comprehensive characterization of CK2 cellular function should firstly address which substrates are phosphorylated exclusively when CK2ß is present (class-III or beta-dependent substrates). However, current experimental constrains limit this classification to a few substrates. Here, we took advantage of motif-based prediction and designed four linear patterns for predicting class-III behavior in sets of experimentally determined CK2 substrates. Integrating high-throughput substrate prediction, functional classification and network analysis, our results suggest that beta-dependent phosphorylation might exert particular regulatory roles in viral infection and biological processes/pathways like apoptosis, DNA repair and RNA metabolism. It also pointed, that human beta-dependent substrates are mainly nuclear, a few of them shuttling between nuclear and cytoplasmic compartments.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Biochem Biophys Rep Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Cuba País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Biochem Biophys Rep Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Cuba País de publicação: Holanda