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Molecular survey of infectious agents associated with bovine respiratory disease in a beef cattle feedlot in southern Brazil.
Headley, Selwyn A; Okano, Werner; Balbo, Luciana C; Marcasso, Rogério A; Oliveira, Thalita E; Alfieri, Alice F; Negri Filho, Luiz C; Michelazzo, Mariana Z; Rodrigues, Silvio C; Baptista, Anderson L; Saut, João Paulo E; Alfieri, Amauri A.
Afiliação
  • Headley SA; Laboratories of Animal Pathology (Headley, Oliveira, Michelazzo), Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paraná, Brazil.
  • Okano W; Molecular Biology (Balbo, AF Alfieri, AA Alfieri), Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paraná, Brazil.
  • Balbo LC; Faculty of Veterinary Medicine, Universidade Norte do Paraná, Arapongas, Paraná, Brazil (Okano, Marcasso, Negri Filho, Rodrigues).
  • Marcasso RA; Large Animal Health Laboratory, Faculty of Veterinary Medicine, Universidade Federal de Uberlândia, Minas Gerais, Brazil (Baptista, Saut).
  • Oliveira TE; Laboratories of Animal Pathology (Headley, Oliveira, Michelazzo), Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paraná, Brazil.
  • Alfieri AF; Molecular Biology (Balbo, AF Alfieri, AA Alfieri), Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paraná, Brazil.
  • Negri Filho LC; Faculty of Veterinary Medicine, Universidade Norte do Paraná, Arapongas, Paraná, Brazil (Okano, Marcasso, Negri Filho, Rodrigues).
  • Michelazzo MZ; Large Animal Health Laboratory, Faculty of Veterinary Medicine, Universidade Federal de Uberlândia, Minas Gerais, Brazil (Baptista, Saut).
  • Rodrigues SC; Laboratories of Animal Pathology (Headley, Oliveira, Michelazzo), Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paraná, Brazil.
  • Baptista AL; Molecular Biology (Balbo, AF Alfieri, AA Alfieri), Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paraná, Brazil.
  • Saut JPE; Faculty of Veterinary Medicine, Universidade Norte do Paraná, Arapongas, Paraná, Brazil (Okano, Marcasso, Negri Filho, Rodrigues).
  • Alfieri AA; Large Animal Health Laboratory, Faculty of Veterinary Medicine, Universidade Federal de Uberlândia, Minas Gerais, Brazil (Baptista, Saut).
J Vet Diagn Invest ; 30(2): 249-251, 2018 Mar.
Article em En | MEDLINE | ID: mdl-29105570
We investigated the occurrence of infectious pathogens during an outbreak of bovine respiratory disease (BRD) in a beef cattle feedlot in southern Brazil that has a high risk of developing BRD. Nasopharyngeal swabs were randomly collected from steers ( n = 23) and assessed for the presence of infectious agents of BRD by PCR and/or RT-PCR assays. These included: Histophilus somni, Mannheimia haemolytica, Pasteurella multocida, Mycoplasma bovis, bovine respiratory syncytial virus (BRSV), bovine coronavirus (BCoV), bovine viral diarrhea virus (BVDV), bovine alphaherpesvirus 1 (BoHV-1), and bovine parainfluenza virus 3 (BPIV-3). Pulmonary sections of one steer that died with clinical BRD were submitted for pathology and molecular testing. The frequencies of the pathogens identified from the nasopharyngeal swabs were: H. somni 39% (9 of 23), BRSV 35% (8 of 23), BCoV 22% (5 of 23), and M. haemolytica 13% (3 of 23). PCR or RT-PCR assays did not identify P. multocida, M. bovis, BoHV-1, BVDV, or BPIV-3 from the nasopharyngeal swabs. Single and concomitant associations of infectious agents of BRD were identified. Fibrinous bronchopneumonia was diagnosed in one steer that died; samples were positive for H. somni and M. haemolytica by PCR. H. somni, BRSV, and BCoV are important disease pathogens of BRD in feedlot cattle in Brazil, but H. somni and BCoV are probably under-reported.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças Respiratórias / Doenças dos Bovinos / Surtos de Doenças Tipo de estudo: Risk_factors_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Vet Diagn Invest Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças Respiratórias / Doenças dos Bovinos / Surtos de Doenças Tipo de estudo: Risk_factors_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Vet Diagn Invest Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos