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Distribution, recovery and concentration of platelets and leukocytes in L-PRP prepared by centrifugation.
de Melo, Bruna Alice Gomes; Martins Shimojo, Andréa Arruda; Marcelino Perez, Amanda Gomes; Duarte Lana, José Fabio Santos; Andrade Santana, Maria Helena.
Afiliação
  • de Melo BAG; Department of Engineering of Materials and Bioprocesses, School of Chemical Engineering, University of Campinas, Campinas-SP, Brazil.
  • Martins Shimojo AA; Department of Engineering of Materials and Bioprocesses, School of Chemical Engineering, University of Campinas, Campinas-SP, Brazil.
  • Marcelino Perez AG; Department of Engineering of Materials and Bioprocesses, School of Chemical Engineering, University of Campinas, Campinas-SP, Brazil.
  • Duarte Lana JFS; Bone and Cartilage Institute, IOC, Indaiatuba-SP, Brazil.
  • Andrade Santana MH; Department of Engineering of Materials and Bioprocesses, School of Chemical Engineering, University of Campinas, Campinas-SP, Brazil. Electronic address: mariahelena.santana@gmail.com.
Colloids Surf B Biointerfaces ; 161: 288-295, 2018 Jan 01.
Article em En | MEDLINE | ID: mdl-29096373
Platelet-rich plasma (PRP) is an autologous product prepared from whole blood (WB) that is widely used in regenerative medicine. In clinical practice, discontinuous centrifugation is used for both hand- and machine-prepared PRP. However, separation of WB fractions via centrifugation is a complex process, and the lack of clear mechanisms limits the understanding and evaluation of PRP preparation methods This paper focuses on the distribution, recovery and concentration factor of platelets and leukocytes in L-PRP (leukocyte and platelet-rich plasma) to define a concentration pattern for these blood components due to centrifugation conditions. WB collected from three healthy donors was centrifuged for 10min at 50-800 xg in a first step and then at 400 xg in a second step. The results from the first centrifugation step showed most platelets to be distributed in the upper layer (UL) and the buffy coat (BC), with approximately 14.5±5.2% retained in the bottom layer (BL). Most leukocytes were present in the BL. The greatest platelet recoveries from L-PRP were obtained at up to 150 xg (88.5±16.9%). The cumulative concentration factors with respect to the WB from the second centrifugation step were 6 and 1.2 for platelets and leukocytes, respectively. Thus, the concentration patterns delineated three centrifugation ranges with platelet/leukocyte ratios of 205±18, 325±15 and 107±4 and lymphocyte/granulocyte ratios of 1.54±0.74, 0.90±0.08 and 0.42±0.07. These findings contribute to a scientifically based standardization of L-PRP preparations.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plaquetas / Separação Celular / Centrifugação / Plasma Rico em Plaquetas / Leucócitos Limite: Humans Idioma: En Revista: Colloids Surf B Biointerfaces Assunto da revista: QUIMICA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plaquetas / Separação Celular / Centrifugação / Plasma Rico em Plaquetas / Leucócitos Limite: Humans Idioma: En Revista: Colloids Surf B Biointerfaces Assunto da revista: QUIMICA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda