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Impact of the Z potential technique on reducing the sperm DNA fragmentation index, fertilization rate and embryo development.
Duarte, Carlos; Núñez, Víctor; Wong, Yat; Vivar, Carlos; Benites, Elder; Rodriguez, Urso; Vergara, Carlos; Ponce, Jorge.
Afiliação
  • Duarte C; NIU VIDA Specialized Center for Assisted Reproduction, Lima, Peru.
  • Núñez V; NIU VIDA Specialized Center for Assisted Reproduction, Lima, Peru.
  • Wong Y; NIU VIDA Specialized Center for Assisted Reproduction, Lima, Peru.
  • Vivar C; Laboratory of Animal Biotechnology, Ricardo Palma University, Lima, Peru.
  • Benites E; NIU VIDA Specialized Center for Assisted Reproduction, Lima, Peru.
  • Rodriguez U; NIU VIDA Specialized Center for Assisted Reproduction, Lima, Peru.
  • Vergara C; NIU VIDA Specialized Center for Assisted Reproduction, Lima, Peru.
  • Ponce J; NIU VIDA Specialized Center for Assisted Reproduction, Lima, Peru.
JBRA Assist Reprod ; 21(4): 351-355, 2017 12 01.
Article em En | MEDLINE | ID: mdl-29072049
BACKGROUND: In assisted reproduction procedures, we need to develop and enhance new protocols to optimize sperm selection. The aim of this study is to evaluate the ability of the Z potential technique to select sperm with intact DNA in non-normospermic patients and evaluate the impact of this selection on embryonic development. METHODS: We analyzed a total of 174 human seminal samples with at least one altered parameter. We measured basal, post density gradients, and post density gradients + Z potential DNA fragmentation index. To evaluate the impact of this technique on embryo development, 54 cases were selected. The embryo development parameters evaluated were fertilization rate, cleavage rate, top quality embryos at the third day and blastocysts rate. RESULTS: We found significant differences in the study groups when we compared the sperm fragmentation index by adding the Z potential technique to density gradient selection vs. density gradients alone. Furthermore, there was no significant difference in the embryo development parameters between the low sperm fragmentation index group vs. the moderate and high sperm fragmentation index groups, when selecting sperms with this new technique. CONCLUSIONS: The Z potential technique is a very useful tool for sperm selection; it significantly reduces the DNA fragmentation index and improves the parameters of embryo development. This technique could be considered routine for its simplicity and low cost.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatozoides / Fertilização in vitro / Injeções de Esperma Intracitoplásmicas / Desenvolvimento Embrionário / Fragmentação do DNA Limite: Adult / Female / Humans / Male / Pregnancy Idioma: En Revista: JBRA Assist Reprod Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Peru País de publicação: Brasil
Texto completo
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatozoides / Fertilização in vitro / Injeções de Esperma Intracitoplásmicas / Desenvolvimento Embrionário / Fragmentação do DNA Limite: Adult / Female / Humans / Male / Pregnancy Idioma: En Revista: JBRA Assist Reprod Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Peru País de publicação: Brasil