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Cut-off value for absolute lymphocytes as an alternative for the immunophenotypic analysis of CD3+ T cells in the monitoring of immunosuppressive therapy with thymoglobulin.
Furlanetto, Gislaine; Alegretti, Ana Paula; Farias, Mariela Granero; Freitas, Priscila Aparecida Corrêa; Lara, Gustavo Muller; Pedrazzani, Fabiane Spagnol.
Afiliação
  • Furlanetto G; Universidade Feevale.
  • Alegretti AP; Hospital de Clínicas de Porto Alegre.
  • Farias MG; Hospital de Clínicas de Porto Alegre.
  • Freitas PAC; Hospital de Clínicas de Porto Alegre.
  • Lara GM; Universidade Feevale.
  • Pedrazzani FS; Hospital de Clínicas de Porto Alegre.
J Bras Nefrol ; 39(2): 181-185, 2017.
Article em En, Pt | MEDLINE | ID: mdl-29069242
INTRODUCTION: Immunosuppression of T lymphocytes is required for preventing acute rejection after transplantation and for the treatment of chronic autoimmune and inflammatory diseases. The laboratory monitoring for this therapy is the measurement of T cells by immunophenotyping, aiming the target value of less than 20 cells per µL. OBJECTIVE: To establish a cut-off point for the total number of lymphocytes in the automated blood cell count that reflects less than twenty T cells µL by immunophenotyping. METHODS: We studied and evaluated 242 kidney transplant patients that had results of automated blood cell count and quantification of T cells by immunophenotyping technique. The patients were divided into two groups, depending on the T lymphocyte immunophenotyping rates established by lower and higher than 20 cells per µL. After, we evaluated the cut-off point for lymphocytes in the blood cell count with a specificity of 100% to exclude patients with high levels of T lymphocytes. RESULTS: We found that the cut-off point of 70 lymphocytes per µL obtained by automated blood cell count showed 100% of specificity to exclude patients with T-cell counts higher than 20 cells per µL by immunophenotyping. CONCLUSION: The results found in this study may be helpful to monitor the immunosuppressive therapy in kidney transplant patients in places where a flow cytometer is not available, or when this equipment is not present in the full routine.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos T / Terapia de Imunossupressão / Transplante de Rim / Complexo CD3 / Imunossupressores / Soro Antilinfocitário Tipo de estudo: Observational_studies Limite: Female / Humans / Male / Middle aged Idioma: En / Pt Revista: J Bras Nefrol Assunto da revista: NEFROLOGIA Ano de publicação: 2017 Tipo de documento: Article País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos T / Terapia de Imunossupressão / Transplante de Rim / Complexo CD3 / Imunossupressores / Soro Antilinfocitário Tipo de estudo: Observational_studies Limite: Female / Humans / Male / Middle aged Idioma: En / Pt Revista: J Bras Nefrol Assunto da revista: NEFROLOGIA Ano de publicação: 2017 Tipo de documento: Article País de publicação: Brasil