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Antimicrobial photodynamic therapy for infectious stomatitis in snakes: Clinical views and microbiological findings.
Grego, Kathleen Fernandes; Carvalho, Marcelo Pires Nogueira de; Cunha, Marcos Paulo Vieira; Knöbl, Terezinha; Pogliani, Fabio Celidonio; Catão-Dias, José Luiz; Sant'Anna, Sávio Stefanini; Ribeiro, Martha Simões; Sellera, Fábio Parra.
Afiliação
  • Grego KF; Laboratory of Herpetology, Butantan Institute, São Paulo/SP, Brazil.
  • Carvalho MPN; Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo/SP, Brazil.
  • Cunha MPV; Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo/SP, Brazil.
  • Knöbl T; Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo/SP, Brazil.
  • Pogliani FC; Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo/SP, Brazil.
  • Catão-Dias JL; Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo/SP, Brazil.
  • Sant'Anna SS; Laboratory of Herpetology, Butantan Institute, São Paulo/SP, Brazil.
  • Ribeiro MS; Center for Lasers and Applications, IPEN-CNEN/SP, São Paulo/SP, Brazil.
  • Sellera FP; Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo/SP, Brazil. Electronic address: fsellera@usp.br.
Photodiagnosis Photodyn Ther ; 20: 196-200, 2017 Dec.
Article em En | MEDLINE | ID: mdl-29037910
BACKGROUND: Antimicrobial photodynamic therapy (APDT) has been broadly investigated as an alternative to treat localized infections, without leading to the selection of resistant microorganisms. Infectious stomatitis is a multifactorial disease frequently reported in captive snakes characterized by infection of the oral mucosa and surrounding tissues. In this study, we investigated methylene blue (MB)-mediated APDT to treat infectious stomatitis in snakes and verified the resistance phenotype and genotype before and after APDT. METHODS: Three Boid snakes presented petechiae, edema and caseous material in their oral cavities. MB (0.01%) was applied on the lesions and after 5min they were irradiated using a red laser (λ=660nm), fluence of 280J/cm2, 8J and 80s per point, 100mW, spot size 0.028cm2 and fluence rate of 3.5W/cm2. APDT was repeated once a week during 3 months. Samples of the lesions were collected to identify bacteria and antibiotic resistance profiles. To analyze the clonality of bacterial isolates before and after APDT, isolates were subjected to ERIC PCR analysis. RESULTS: Snakes presented clinical improvement such as reduction of inflammatory signs and caseous material. Pseudomonas aeruginosa and Escherichia coli were present in all snakes; Klebsiella pneumoniae and Morganella morganii were also identified in some animals. We also observed that the oral microbiota was completely replaced following APDT. However, K. pneumoniae isolates before and after APDT were a single clone with 100% of genetic similarity that lost resistance phenotype for seven antibiotics of four classes. CONCLUSIONS: These results show that APDT can be used to treat infectious stomatitis in snakes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Dermatopatias / Fármacos Fotossensibilizantes / Azul de Metileno Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Photodiagnosis Photodyn Ther Assunto da revista: DIAGNOSTICO POR IMAGEM / TERAPEUTICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Dermatopatias / Fármacos Fotossensibilizantes / Azul de Metileno Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Photodiagnosis Photodyn Ther Assunto da revista: DIAGNOSTICO POR IMAGEM / TERAPEUTICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda