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Viability, purity, and genetic stability of entomopathogenic fungi species using different preservation methods.
Ayala-Zermeño, Miguel A; Gallou, Adrien; Berlanga-Padilla, Angélica M; Andrade-Michel, Gilda Y; Rodríguez-Rodríguez, José C; Arredondo-Bernal, Hugo C; Montesinos-Matías, Roberto.
Afiliação
  • Ayala-Zermeño MA; Centro Nacional de Referencia de Control Biológico, SENASICA-DGSV-CNRF, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico.
  • Gallou A; Centro Nacional de Referencia de Control Biológico, SENASICA-DGSV-CNRF, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico.
  • Berlanga-Padilla AM; Centro Nacional de Referencia de Control Biológico, SENASICA-DGSV-CNRF, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico.
  • Andrade-Michel GY; Centro Nacional de Referencia de Control Biológico, SENASICA-DGSV-CNRF, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico.
  • Rodríguez-Rodríguez JC; Centro Nacional de Referencia de Control Biológico, SENASICA-DGSV-CNRF, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico.
  • Arredondo-Bernal HC; Centro Nacional de Referencia de Control Biológico, SENASICA-DGSV-CNRF, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico.
  • Montesinos-Matías R; Centro Nacional de Referencia de Control Biológico, SENASICA-DGSV-CNRF, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico. Electronic address: montesinosroberto@yahoo.com.mx.
Fungal Biol ; 121(11): 920-928, 2017 11.
Article em En | MEDLINE | ID: mdl-29029699
Preservation methods for entomopathogenic fungi (EPF) require effective protocols to ensure uniform processes and to avoid alterations during storage. The aim of this study was to preserve Beauveria bassiana, Metarhizium acridum, M. anisopliae, M. rileyi, Isaria javanica, Hirsutella thompsonii, H. citriformis and Lecanicillium lecanii in mineral oil (MO), sterile water (SW), silica gel (SG), lyophilisation (L), ultracold-freezing at -70 °C, and cryopreservation at -196 °C. The viability and purity of the fungi were then verified: phenotypic characteristics were evaluated qualitatively at 6, 12 and 24 m. Genetic stability was tested by amplified fragment length polymorphisms (AFLP) analysis at 24 m. Of the eight species of EPF, three remained viable in SW, five in MO and L, six at -70 °C, seven in SG, and eight at -196 °C. No significant changes were observed in AFLP patterns at 24 m of storage. The most effective preservation methods for EPF were SG, L, -70 and -196 °C. Beauveria bassiana, M. acridum, M. anisopliae, M. rileyi and I. javanica remained stable with all methods, while the remaining species were less compatible. The optimisation of preservation methods for EPF facilitates the development of reliable protocols to ensure their inherent characteristics in culture collections.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Preservação Biológica / Hypocreales Idioma: En Revista: Fungal Biol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: México País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Preservação Biológica / Hypocreales Idioma: En Revista: Fungal Biol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: México País de publicação: Holanda