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Suppressor of cytokine signaling 1 expression during LPS-induced inflammation and bone loss in rats.
Souza, João Antonio Chaves de; Nogueira, Andressa Vilas Boas; Souza, Pedro Paulo Chaves de; Oliveira, Guilherme José Pimentel Lopes de; Medeiros, Marcell Costa de; Garlet, Gustavo Pompermaier; Cirelli, Joni Augusto; Rossa, Carlos.
Afiliação
  • Souza JAC; Universidade Federal de Goiás - UFG, Faculty of Dentistry, Department of Periodontology, Goiânia, GO, Brazil.
  • Nogueira AVB; Universidade Estadual Paulista - UNESP, School of Dentistry at Araraquara, Department of Diagnosis and Surgery, Araraquara, SP, Brazil.
  • Souza PPC; Universidade Estadual Paulista - UNESP, School of Dentistry at Araraquara, Department of Physiology and Pathology, Araraquara, SP, Brazil.
  • Oliveira GJPL; Universidade Estadual Paulista - UNESP, School of Dentistry at Araraquara, Department of Diagnosis and Surgery, Araraquara, SP, Brazil.
  • Medeiros MC; Universidade Estadual Paulista - UNESP, School of Dentistry at Araraquara, Department of Diagnosis and Surgery, Araraquara, SP, Brazil.
  • Garlet GP; Universidade de São Paulo - USP, School of Dentistry at Bauru, Department of Biological Sciences, Bauru, SP, Brazil.
  • Cirelli JA; Universidade Estadual Paulista - UNESP, School of Dentistry at Araraquara, Department of Diagnosis and Surgery, Araraquara, SP, Brazil.
  • Rossa C; Universidade Estadual Paulista - UNESP, School of Dentistry at Araraquara, Department of Diagnosis and Surgery, Araraquara, SP, Brazil.
Braz Oral Res ; 31: e75, 2017 Sep 28.
Article em En | MEDLINE | ID: mdl-29019549
This study aimed to characterize the dynamics of suppressor of cytokine signaling (SOCS1) expression in a rat model of lipopolysaccharide-induced periodontitis. Wistar rats in the experimental groups were injected three times/week with LPS from Escherichia coli on the palatal aspect of the first molars, and control animals were injected with vehicle (phosphate-buffered saline). Animals were sacrificed 7, 15, and 30 days after the first injection to analyze inflammation (stereometric analysis), bone loss (macroscopic analysis), gene expression (qRT-PCR), and protein expression/activation (Western blotting). The severity of inflammation and bone loss associated with LPS-induced periodontitis increased from day 7 to day 15, and it was sustained through day 30. Significant (p < 0.05) increases in SOCS1, RANKL, OPG, and IFN-γ gene expression were observed in the experimental group versus the control group at day 15. SOCS1 protein expression and STAT1 and NF-κB activation were increased throughout the 30-day experimental period. Gingival tissues affected by experimental periodontitis express SOCS1, indicating that this protein may potentially downregulate signaling events involved in inflammatory reactions and bone loss and thus may play a relevant role in the development and progression of periodontal disease.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Periodontite / Perda do Osso Alveolar / Proteína 1 Supressora da Sinalização de Citocina Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Braz Oral Res Assunto da revista: ODONTOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Periodontite / Perda do Osso Alveolar / Proteína 1 Supressora da Sinalização de Citocina Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Braz Oral Res Assunto da revista: ODONTOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil