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Influence of the abstinence period on human sperm quality: analysis of 2,458 semen samples.
Comar, Vanessa A; Petersen, Claudia G; Mauri, Ana L; Mattila, Mariana; Vagnini, Laura D; Renzi, Adriana; Petersen, Bruna; Nicoletti, Andreia; Dieamant, Felipe; Oliveira, Joao Batista A; Baruffi, Ricardo L R; Franco, José G.
Afiliação
  • Comar VA; Center for Human Reproduction Prof. Franco Jr., Ribeirão Preto, SP, Brazil.
  • Petersen CG; Center for Human Reproduction Prof. Franco Jr., Ribeirão Preto, SP, Brazil.
  • Mauri AL; Paulista Center for Diagnosis Research and Training, Ribeirão Preto, SP, Brazil.
  • Mattila M; Center for Human Reproduction Prof. Franco Jr., Ribeirão Preto, SP, Brazil.
  • Vagnini LD; Paulista Center for Diagnosis Research and Training, Ribeirão Preto, SP, Brazil.
  • Renzi A; Center for Human Reproduction Prof. Franco Jr., Ribeirão Preto, SP, Brazil.
  • Petersen B; Paulista Center for Diagnosis Research and Training, Ribeirão Preto, SP, Brazil.
  • Nicoletti A; Paulista Center for Diagnosis Research and Training, Ribeirão Preto, SP, Brazil.
  • Dieamant F; Paulista Center for Diagnosis Research and Training, Ribeirão Preto, SP, Brazil.
  • Oliveira JBA; Center for Human Reproduction Prof. Franco Jr., Ribeirão Preto, SP, Brazil.
  • Baruffi RLR; Center for Human Reproduction Prof. Franco Jr., Ribeirão Preto, SP, Brazil.
  • Franco JG; Paulista Center for Diagnosis Research and Training, Ribeirão Preto, SP, Brazil.
JBRA Assist Reprod ; 21(4): 306-312, 2017 12 01.
Article em En | MEDLINE | ID: mdl-28985041
OBJECTIVE: The aim of this study was to evaluate the influence of different periods of abstinence on conventional semen parameters as well as functional parameters in human semen, including mitochondrial function, chromatin packing and sperm DNA fragmentation. METHODS: We recruited a cohort of 2,458 men undergoing infertility investigation. Semen analyses were performed according to WHO guidelines/morphology-motile sperm organelle morphology examination/MSOME. For DNA integrity analysis, the percentages of DNA fragmentation (TUNEL), abnormal chromatin packaging/underprotamination (chromomycin A3/CMA3), abnormal mitochondrial membrane potential (MMP/MitoTracker Green), and apoptosis (annexin-V) were recorded. Associations between the sexual abstinence period and sperm parameters were assessed using Spearman correlation. For group comparisons, the subjects were categorized according to the sexual abstinence period (SAP) into three groups: SAP <2 days, SAP 2-5 days, and SAP >5 days. RESULTS: The duration of abstinence had a statistically significant positive influence on sperm concentration and volume, the number of leukocytes and a statistically significant negative influence on sperm motility and vitality. The percentages of DNA fragmentation and MMP (mitochondrial damage) worsened with the increased duration of abstinence. The percentage of sperm protamination was statistically significantly increased with abstinence. CONCLUSION: Increase in the sexual abstinence period influences sperm quality. This study reinforces the importance of the duration of ejaculatory abstinence on semen parameter variation. It highlights the deleterious effect of increased abstinence on DNA damage, which is most likely associated with ROS (mitochondrial damage/number of leukocytes). The increase in chromatin packaging can represent a protective feature for DNA.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Abstinência Sexual / Motilidade dos Espermatozoides / Espermatozoides / Fragmentação do DNA Limite: Adult / Humans / Male Idioma: En Revista: JBRA Assist Reprod Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Abstinência Sexual / Motilidade dos Espermatozoides / Espermatozoides / Fragmentação do DNA Limite: Adult / Humans / Male Idioma: En Revista: JBRA Assist Reprod Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil