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Detection of Colistin-Resistant MCR-1-Positive Escherichia coli by Use of Assays Based on Inhibition by EDTA and Zeta Potential.
Esposito, Fernanda; Fernandes, Miriam R; Lopes, Ralf; Muñoz, Maria; Sabino, Caetano P; Cunha, Marcos P; Silva, Ketrin C; Cayô, Rodrigo; Martins, Willames M B S; Moreno, Andrea M; Knöbl, Terezinha; Gales, Ana C; Lincopan, Nilton.
Afiliação
  • Esposito F; Department of Clinical Analysis, School of Pharmacy, University de São Paulo, São Paulo, Brazil.
  • Fernandes MR; Department of Clinical Analysis, School of Pharmacy, University de São Paulo, São Paulo, Brazil.
  • Lopes R; Department of Microbiology, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil.
  • Muñoz M; Department of Clinical Analysis, School of Pharmacy, University de São Paulo, São Paulo, Brazil.
  • Sabino CP; Department of Clinical Analysis, School of Pharmacy, University de São Paulo, São Paulo, Brazil.
  • Cunha MP; School of Veterinary Medicine, Universidade de São Paulo, São Paulo, Brazil.
  • Silva KC; School of Veterinary Medicine, Universidade de São Paulo, São Paulo, Brazil.
  • Cayô R; Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
  • Martins WMBS; Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
  • Moreno AM; School of Veterinary Medicine, Universidade de São Paulo, São Paulo, Brazil.
  • Knöbl T; School of Veterinary Medicine, Universidade de São Paulo, São Paulo, Brazil.
  • Gales AC; Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
  • Lincopan N; Department of Clinical Analysis, School of Pharmacy, University de São Paulo, São Paulo, Brazil lincopan@usp.br.
J Clin Microbiol ; 55(12): 3454-3465, 2017 12.
Article em En | MEDLINE | ID: mdl-28978685
The emergence and rapid dissemination of colistin-resistant Escherichia coli carrying the plasmid-mediated mcr-1 gene have created an urgent need to develop specific screening methods. In this study, we evaluated four assays based on the inhibition of MCR-1 activity by EDTA: (i) a combined-disk test (CDT) comparing the inhibition zones of colistin and colistin (10 µg) plus EDTA (100 mM); (ii) reduction of colistin MIC (CMR) in the presence of EDTA (80 µg/ml); (iii) a modified rapid polymyxin Nordmann/Poirel test (MPNP); and (iv) alteration of zeta potential (RZP = ZP+EDTA/ZP-EDTA). We obtained encouraging results for the detection of MCR-1 in E. coli isolates recovered from human, food, and animal samples, using the following assay parameters: ≥3 mm difference in the inhibition zones between colistin disks without and with EDTA; ≥4-fold colistin MIC decrease in the presence of EDTA; RZP of ≥2.5; and the absence of metabolic activity and proliferation, indicated by unchanged color of phenol red in the presence of colistin-EDTA, in the MPNP test. In this regard, the CDT, CMR, RZP, and MPNP assays exhibited sensitivities of 96.7, 96.7, 95.1, and 96.7% and specificities of 89.6, 83.3, 100, and 100%, respectively, for detecting MCR-1-positive E. coli Our results demonstrate that inhibition by EDTA and zeta potential assays may provide simple and inexpensive methods for the presumptive detection of MCR-1-producing E. coli isolates in human and veterinary diagnostic laboratories.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Testes de Sensibilidade Microbiana / Colistina / Proteínas de Escherichia coli / Farmacorresistência Bacteriana / Escherichia coli / Antibacterianos Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Animals / Humans Idioma: En Revista: J Clin Microbiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Testes de Sensibilidade Microbiana / Colistina / Proteínas de Escherichia coli / Farmacorresistência Bacteriana / Escherichia coli / Antibacterianos Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Animals / Humans Idioma: En Revista: J Clin Microbiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos