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Antibacterial activity of a novel antimicrobial peptide [W7]KR12-KAEK derived from KR-12 against Streptococcus mutans planktonic cells and biofilms.
da Silva, B R; Conrado, A J S; Pereira, A L; Evaristo, F F V; Arruda, F V S; Vasconcelos, M A; Lorenzón, E N; Cilli, E M; Teixeira, E H.
Afiliação
  • da Silva BR; a DPML/LIBS, Integrated Laboratory of Biomolecules , Federal University of Ceará , Fortaleza , Brazil.
  • Conrado AJS; d School of Dentistry , Universidade de Fortaleza - UNIFOR , Fortaleza , Brazil.
  • Pereira AL; a DPML/LIBS, Integrated Laboratory of Biomolecules , Federal University of Ceará , Fortaleza , Brazil.
  • Evaristo FFV; a DPML/LIBS, Integrated Laboratory of Biomolecules , Federal University of Ceará , Fortaleza , Brazil.
  • Arruda FVS; a DPML/LIBS, Integrated Laboratory of Biomolecules , Federal University of Ceará , Fortaleza , Brazil.
  • Vasconcelos MA; b School of Dentistry , Faculdades INTA , Sobral , Brazil.
  • Lorenzón EN; a DPML/LIBS, Integrated Laboratory of Biomolecules , Federal University of Ceará , Fortaleza , Brazil.
  • Cilli EM; b School of Dentistry , Faculdades INTA , Sobral , Brazil.
  • Teixeira EH; a DPML/LIBS, Integrated Laboratory of Biomolecules , Federal University of Ceará , Fortaleza , Brazil.
Biofouling ; 33(10): 835-846, 2017 11.
Article em En | MEDLINE | ID: mdl-28967271
The aims of this study were to describe the synthesis of a novel synthetic peptide based on the primary structure of the KR-12 peptide and to evaluate its antimicrobial and anti-biofilm activities against Streptococcus mutans. The antimicrobial effect of KR-12 and [W7]KR12-KAEK was assessed by determining the minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations. The evaluation of anti-biofilm activity was assessed through total biomass quantification, colony forming unit counting and scanning electron microscopy. [W7]KR12-KAEK showed MIC and MBC values ranging from 31.25 to 7.8 and 62.5 to 15.6 µg ml-1, respectively. Furthermore, [W7]KR12-KAEK significantly reduced biofilm biomass (50-100%). Regarding cell viability, [W7]KR12-KAEK showed reductions in the number of CFUs at concentrations ranging from 62.5 to 7.8 µg ml-1 and 500 to 62.5 µg ml-1 with respect to biofilm formation and preformed biofilms, respectively. SEM micrographs of S. mutans treated with [W7]KR12-KAEK suggested damage to the bacterial surface. [W7]KR12-KAEK is demonstrated to be an antimicrobial agent to control microbial biofilms.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptococcus mutans / Catelicidinas / Antibacterianos Idioma: En Revista: Biofouling Assunto da revista: BIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptococcus mutans / Catelicidinas / Antibacterianos Idioma: En Revista: Biofouling Assunto da revista: BIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido