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Evaluation of mutagenic and antimicrobial properties of brown propolis essential oil from the Brazilian Cerrado biome.
Fernandes, Fábio H; da R Guterres, Zaira; Violante, Ivana M P; Lopes, Tiago F S; Garcez, Walmir S; Garcez, Fernanda R.
Afiliação
  • Fernandes FH; Instituto de Química, Universidade Federal de Mato Grosso do Sul, Av. Senador Filinto Muller 1555, Campo Grande, MS 79074-460, Brazil.
  • da R Guterres Z; Universidade Estadual de Mato Grosso do Sul, Unidade Universitária de Mundo Novo, BR163, km 202, Mundo Novo, MS 79980-000, Brazil.
  • Violante IMP; Departamento de Farmácia, Universidade de Cuiabá, Av. Beira Rio 3100, Cuiabá, MT 78015-480, Brazil.
  • Lopes TFS; Universidade Estadual de Mato Grosso do Sul, Unidade Universitária de Mundo Novo, BR163, km 202, Mundo Novo, MS 79980-000, Brazil.
  • Garcez WS; Instituto de Química, Universidade Federal de Mato Grosso do Sul, Av. Senador Filinto Muller 1555, Campo Grande, MS 79074-460, Brazil.
  • Garcez FR; Instituto de Química, Universidade Federal de Mato Grosso do Sul, Av. Senador Filinto Muller 1555, Campo Grande, MS 79074-460, Brazil.
Toxicol Rep ; 2: 1482-1488, 2015.
Article em En | MEDLINE | ID: mdl-28962491
Biological, and particularly antimicrobial, activities have been demonstrated for the essential oil of propolis samples worlwide, yet their mutagenic effects remain unknown. To correlate antimicrobial effects with mutagenic risks, the present study evaluated the antifungal and antibacterial activities of the essential oil obtained from brown propolis collected from the Cerrado biome in Midwest Brazil (EOP), testing it against nine pathogenic microorganisms. Evaluation of mutagenic potential was based on the somatic mutation and recombination test (SMART) performed on wing cells of standard (ST) and high-bioactivation (HB) crosses of Drosophila melanogaster. EOP was extracted by hydrodistillation, and sesquiterpenes were characterized by GCâ¿¿MS as its major constituents. The crude oil proved active against Cryptococcus neoformans and Enterococcus faecalis, as did two of its major constituents, spathulenol and (E)-nerolidol â¿¿ the latter being also active against Staphylococcus aureus â¿¿ isolated using chromatographic procedures. No significant increase in the number of somatic mutations was observed in the offspring of ST or HB crosses â¿¿ the latter exhibiting enhanced levels of metabolizing enzymes of the cytochrome P450 type â¿¿ treated with 0.05%, 0.1%, and 0.2% EOP. These findings revealed no mutagenic activity of EOP, even when tested against the HB strain, and demonstrated that its antimicrobial activities are not associated with DNA damage induction (investigated with SMART), suggesting the potential of EOP as a natural preservative.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE País/Região como assunto: America do sul / Brasil Idioma: En Revista: Toxicol Rep Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irlanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE País/Região como assunto: America do sul / Brasil Idioma: En Revista: Toxicol Rep Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irlanda