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On-line monitoring of Aspergillus niger GH1 growth in a bioprocess for the production of ellagic acid and ellagitannase by solid-state fermentation.
Aguilar-Zárate, Pedro; Wong-Paz, Jorge E; Rodríguez-Duran, Luis V; Buenrostro-Figueroa, Juan; Michel, Mariela; Saucedo-Castañeda, Gerardo; Favela-Torres, Ernesto; Ascacio-Valdés, Juan A; Contreras-Esquivel, Juan C; Aguilar, Cristóbal N.
Afiliação
  • Aguilar-Zárate P; Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, 25280 Saltillo, Coahuila, Mexico; Instituto Tecnológico de Ciudad Valles, Tecnológico Nacional de México, 79010, Ciudad Valles, San Luis Potosí, Mexico.
  • Wong-Paz JE; Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, 25280 Saltillo, Coahuila, Mexico; Instituto Tecnológico de Ciudad Valles, Tecnológico Nacional de México, 79010, Ciudad Valles, San Luis Potosí, Mexico.
  • Rodríguez-Duran LV; Department of Biotechnology, Universidad Autónoma Metropolitana Iztapalapa, 09340 Mexico City, Mexico.
  • Buenrostro-Figueroa J; Department of Biotechnology, Universidad Autónoma Metropolitana Iztapalapa, 09340 Mexico City, Mexico.
  • Michel M; Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, 25280 Saltillo, Coahuila, Mexico.
  • Saucedo-Castañeda G; Department of Biotechnology, Universidad Autónoma Metropolitana Iztapalapa, 09340 Mexico City, Mexico.
  • Favela-Torres E; Department of Biotechnology, Universidad Autónoma Metropolitana Iztapalapa, 09340 Mexico City, Mexico.
  • Ascacio-Valdés JA; Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, 25280 Saltillo, Coahuila, Mexico.
  • Contreras-Esquivel JC; Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, 25280 Saltillo, Coahuila, Mexico.
  • Aguilar CN; Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, 25280 Saltillo, Coahuila, Mexico. Electronic address: cristobal.aguilar@uadec.edu.mx.
Bioresour Technol ; 247: 412-418, 2018 Jan.
Article em En | MEDLINE | ID: mdl-28961447
The present work describes the monitoring of CO2 production by Aspergillus niger GH1 in a bioprocess for the production of ellagitannase (EAH) and ellagic acid by solid state fermentation. Pomegranate ellagitannins, mainly punicalagin, were used as carbon source and EAH inducer. A second condition, using ellagitannins and maltose as growth promoting carbon source, was tested. The ellagic acid production was quantified and the EAH activity was assayed. The accumulated metabolites were identified by HPLC-ESI-MS/MS. Higher CO2 production (7.79mg/grams of dry material) was reached in media supplemented with maltose. Short-time lag phase (7.79h) and exponential phase (10.42h) were obtained using only ellagitannins, despite its lower CO2 production (3.79mg/grams of dry material). Without the use of maltose lower ellagic acid (11.85mg/L/h) and EAH (21.80U/L/h) productivities were reached. The use of maltose enhances the productivity of EA (33.18mg/L/h) and EAH (33.70U/L/h). Besides of punicalin and ellagic acid, two unknown compounds with mass weight of 702 and 290g/mol (ions 701 and 289m/z in negative mode, respectively) were identified and characterized by HPLC-ESI-MS/MS analysis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aspergillus niger / Ácido Elágico / Fermentação Idioma: En Revista: Bioresour Technol Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aspergillus niger / Ácido Elágico / Fermentação Idioma: En Revista: Bioresour Technol Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido