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Accelerated Evolution and Functional Divergence of the Dim Light Visual Pigment Accompanies Cichlid Colonization of Central America.
Hauser, Frances E; Ilves, Katriina L; Schott, Ryan K; Castiglione, Gianni M; López-Fernández, Hernán; Chang, Belinda S W.
Afiliação
  • Hauser FE; Department of Ecology and Evolutionary Biology, University of Toronto, Toronto, ON, Canada.
  • Ilves KL; Department of Natural History, Royal Ontario Museum, Toronto, ON, Canada.
  • Schott RK; Department of Biology, Pace University, New York, NY.
  • Castiglione GM; Department of Ecology and Evolutionary Biology, University of Toronto, Toronto, ON, Canada.
  • López-Fernández H; Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada.
  • Chang BSW; Department of Ecology and Evolutionary Biology, University of Toronto, Toronto, ON, Canada.
Mol Biol Evol ; 34(10): 2650-2664, 2017 10 01.
Article em En | MEDLINE | ID: mdl-28957507
Cichlids encompass one of the most diverse groups of fishes in South and Central America, and show extensive variation in life history, morphology, and colouration. While studies of visual system evolution in cichlids have focussed largely on the African rift lake species flocks, Neotropical cichlids offer a unique opportunity to investigate visual system evolution at broader temporal and geographic scales. South American cichlid colonization of Central America has likely promoted accelerated rates of morphological evolution in Central American lineages as they encountered reduced competition, renewed ecological opportunity, and novel aquatic habitats. To investigate whether such transitions have influenced molecular evolution of vision in Central American cichlids, we sequenced the dim-light rhodopsin gene in 101 Neotropical cichlid species, spanning the diversity of the clade. We find strong evidence for increased rates of evolution in Central American cichlid rhodopsin relative to South American lineages, and identify several sites under positive selection in rhodopsin that likely contribute to adaptation to different photic environments. We expressed a Neotropical cichlid rhodopsin protein invitro for the first time, and found that while its spectral tuning properties were characteristic of typical vertebrate rhodopsin pigments, the rate of decay of its active signalling form was much slower, consistent with dim light adaptation in other vertebrate rhodopsins. Using site-directed mutagenesis combined with spectroscopic assays, we found that a key amino acid substitution present in some Central American cichlids accelerates the rate of decay of active rhodopsin, which may mediate adaptation to clear water habitats.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rodopsina / Ciclídeos / Adaptação à Escuridão Tipo de estudo: Prognostic_studies Limite: Animals País/Região como assunto: America central Idioma: En Revista: Mol Biol Evol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Canadá País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rodopsina / Ciclídeos / Adaptação à Escuridão Tipo de estudo: Prognostic_studies Limite: Animals País/Região como assunto: America central Idioma: En Revista: Mol Biol Evol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Canadá País de publicação: Estados Unidos