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Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis.
Magalhães, Franklin B; Castro Neto, Artur L; Nascimento, Marilia B; Santos, Wagner J T; Medeiros, Zulma M; Lima Neto, Adelino S; Costa, Dorcas L; Costa, Carlos H N; Dos Santos, Washington L C; Pontes de Carvalho, Lain C; Oliveira, Geraldo G S; de Melo Neto, Osvaldo P.
Afiliação
  • Magalhães FB; Associação Caruaruense de Ensino Superior e Técnico, Caruaru, Pernambuco, Brazil.
  • Castro Neto AL; Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz (Fiocruz-Pernambuco), Recife, Pernambuco, Brazil.
  • Nascimento MB; Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz (Fiocruz-Pernambuco), Recife, Pernambuco, Brazil.
  • Santos WJT; Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz (Fiocruz-Pernambuco), Recife, Pernambuco, Brazil.
  • Medeiros ZM; Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz (Fiocruz-Pernambuco), Recife, Pernambuco, Brazil.
  • Lima Neto AS; Instituto de Doenças Tropicais Natan Portella (IDTNP), Teresina, Piaui, Brazil.
  • Costa DL; Instituto de Doenças Tropicais Natan Portella (IDTNP), Teresina, Piaui, Brazil.
  • Costa CHN; Instituto de Doenças Tropicais Natan Portella (IDTNP), Teresina, Piaui, Brazil.
  • Dos Santos WLC; Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz (Fiocruz-Bahia), Salvador, Bahia, Brazil.
  • Pontes de Carvalho LC; Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz (Fiocruz-Bahia), Salvador, Bahia, Brazil.
  • Oliveira GGS; Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz (Fiocruz-Bahia), Salvador, Bahia, Brazil.
  • de Melo Neto OP; Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz (Fiocruz-Pernambuco), Recife, Pernambuco, Brazil.
PLoS One ; 12(9): e0184867, 2017.
Article em En | MEDLINE | ID: mdl-28957332
Current strategies for the control of zoonotic visceral leishmaniasis (VL) rely on its efficient diagnosis in both human and canine hosts. The most promising and cost effective approach is based on serologic assays with recombinant proteins. However, no single antigen has been found so far which can be effectively used to detect the disease in both dogs and humans. In previous works, we identified Leishmania infantum antigens with potential for the serodiagnosis of VL. Here, we aimed to expand the panel of the available antigens for VL diagnosis through another screening of a genomic expression library. Seven different protein-coding gene fragments were identified, five of which encoding proteins which have not been previously studied in Leishmania and rich in repetitive motifs. Poly-histidine tagged polypeptides were generated from six genes and evaluated for their potential for diagnosis of VL by ELISA (Enzyme Linked ImmunoSorbent Assay) with sera from infected humans and dogs. None of those was valid for the detection of human VL (26-52% sensitivity) although their performance was increased in the canine sera (48-91% sensitivity), with one polypeptide useful for the diagnosis of canine leishmaniasis. Next, we assayed a mixture of three antigens, found to be best for human or canine VL, among 13 identified through different screenings. This "Mix" resulted in similar levels of sensitivity for both human (84%) and canine (88%) sera. With improvements, this validates the use of multiple proteins, including antigens identified here, as components of a single system for the diagnosis of both forms of leishmaniasis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes / Testes Sorológicos / Leishmaniose Visceral / Antígenos de Protozoários Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes / Testes Sorológicos / Leishmaniose Visceral / Antígenos de Protozoários Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos