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The regulatory role of IL-6R in hepatitis B-associated fibrosis and cirrhosis.
Chen, Y; Yang, S; Peng, Y; Yang, Z.
Afiliação
  • Chen Y; Department of Infectious Diseases, Linyi People's Hospital, Linyi, Shandong, China.
  • Yang S; Department of Infectious Diseases, Linyi People's Hospital, Linyi, Shandong, China.
  • Peng Y; Department of Infectious Diseases, Linyi People's Hospital, Linyi, Shandong, China.
  • Yang Z; Department of Infectious Diseases, Linyi People's Hospital, Linyi, Shandong, China.
Braz J Med Biol Res ; 50(11): e6246, 2017 Sep 21.
Article em En | MEDLINE | ID: mdl-28953986
This study investigated the expression and regulation of IL-6R in hepatitis B-associated moderate hepatic fibrosis and cirrhosis. Liver tissues, peripheral blood monocytes (PBMs) and serum were collected from 26 hepatitis B patients with liver fibrosis and 35 hepatitis B patients with liver cirrhosis. The levels of Il-6r mRNA expression in these samples were examined by quantitative real-time PCR and IL-6R protein levels were analyzed by western blot and ELISA. MiRNAs that regulate IL-6R expression were predicted by bioinformatics analysis, and validated by dual luciferase reporter assay. Compared with the hepatic fibrosis group, IL-6R was significantly upregulated at both mRNA and protein levels in liver tissues, PBMs and serum samples from the hepatic cirrhosis group (P<0.05). The 3'UTR of Il-6r mRNA was predicted to contain a miR-30b binding site and IL-6R was identified as a possible target of miR-30b. MiR-30b expression was significantly downregulated in samples from hepatic cirrhosis patients compared with hepatic fibrosis patients (P<0.05). In conclusion, IL-6R was upregulated while miR-30b was decreased in patients with liver cirrhosis. The miR-30 can directly regulate the expression of IL-6R.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores de Interleucina-6 / MicroRNAs / Hepatite B / Cirrose Hepática Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores de Interleucina-6 / MicroRNAs / Hepatite B / Cirrose Hepática Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China País de publicação: Brasil