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Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi: Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes.
Huarte-Bonnet, Carla; Kumar, Suresh; Saparrat, Mario C N; Girotti, Juan R; Santana, Marianela; Hallsworth, John E; Pedrini, Nicolás.
Afiliação
  • Huarte-Bonnet C; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad Nacional de La Plata (UNLP), calles 60 y 120, 1900, La Plata, Argentina.
  • Kumar S; Department of Diagnostic and Allied Health Sciences, Faculty of Health and Life Sciences, Management and Science University, University Drive, Seksyen 13, 40100, Shah Alam, Selangor, Malaysia.
  • Saparrat MCN; Instituto de Fisiología Vegetal (INFIVE), Universidad Nacional de La Plata (UNLP)-CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) Diag. 113 y 61, CC 327, 1900, La Plata, Argentina.
  • Girotti JR; Cátedra de Microbiología Agrícola, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 119, 1900, La Plata, Argentina.
  • Santana M; Instituto de Botánica Spegazzini, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, 53 # 477, 1900, La Plata, Argentina.
  • Hallsworth JE; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad Nacional de La Plata (UNLP), calles 60 y 120, 1900, La Plata, Argentina.
  • Pedrini N; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CCT La Plata Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad Nacional de La Plata (UNLP), calles 60 y 120, 1900, La Plata, Argentina.
Appl Biochem Biotechnol ; 184(3): 1047-1060, 2018 Mar.
Article em En | MEDLINE | ID: mdl-28942502
Several filamentous fungi are able to concomitantly assimilate both aliphatic and polycyclic aromatic hydrocarbons that are the biogenic by-products of some industrial processes. Cytochrome P450 monooxygenases catalyze the first oxidation reaction for both types of substrate. Among the cytochrome P450 (CYP) genes, the family CYP52 is implicated in the first hydroxylation step in alkane-assimilation processes, while genes belonging to the family CYP53 have been linked with oxidation of aromatic hydrocarbons. Here, we perform a comparative analysis of CYP genes belonging to clans CYP52 and CYP53 in Aspergillus niger, Beauveria bassiana, Metarhizium robertsii (formerly M. anisopliae var. anisopliae), and Penicillium chrysogenum. These species were able to assimilate n-hexadecane, n-octacosane, and phenanthrene, exhibiting a species-dependent modification in pH of the nutrient medium during this process. Modeling of the molecular docking of the hydrocarbons to the cytochrome P450 active site revealed that both phenanthrene and n-octacosane are energetically favored as substrates for the enzymes codified by genes belonging to both CYP52 and CYP53 clans, and thus appear to be involved in this oxidation step. Analyses of gene expression revealed that CYP53 members were significantly induced by phenanthrene in all species studied, but only CYP52X1 and CYP53A11 from B. bassiana were highly induced with n-alkanes. These findings suggest that the set of P450 enzymes involved in hydrocarbon assimilation by fungi is dependent on phylogeny and reveal distinct substrate and expression specificities.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Fúngicas / Sistema Enzimático do Citocromo P-450 / Eurotiales / Hidrocarbonetos Cíclicos / Hypocreales Tipo de estudo: Prognostic_studies Idioma: En Revista: Appl Biochem Biotechnol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Fúngicas / Sistema Enzimático do Citocromo P-450 / Eurotiales / Hidrocarbonetos Cíclicos / Hypocreales Tipo de estudo: Prognostic_studies Idioma: En Revista: Appl Biochem Biotechnol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos