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Antibacterial Activity of Endophytic Actinomycetes Isolated from the Medicinal Plant Vochysia divergens (Pantanal, Brazil).
Gos, Francielly M W R; Savi, Daiani C; Shaaban, Khaled A; Thorson, Jon S; Aluizio, Rodrigo; Possiede, Yvelise M; Rohr, Jürgen; Glienke, Chirlei.
Afiliação
  • Gos FMWR; Department of Basic Pathology, Federal University of ParanáCuritiba, Brazil.
  • Savi DC; Department of Genetics, Federal University of ParanáCuritiba, Brazil.
  • Shaaban KA; Department of Pharmaceutical Sciences, College of Pharmacy, University of KentuckyLexington, KY, United States.
  • Thorson JS; Center for Pharmaceutical Research and Innovation, College of Pharmacy, University of KentuckyLexington, KY, United States.
  • Aluizio R; Department of Pharmaceutical Sciences, College of Pharmacy, University of KentuckyLexington, KY, United States.
  • Possiede YM; Center for Pharmaceutical Research and Innovation, College of Pharmacy, University of KentuckyLexington, KY, United States.
  • Rohr J; Department of Genetics, Federal University of ParanáCuritiba, Brazil.
  • Glienke C; Department of Biology, Federal University of Mato Grosso do SulCampo Grande, Brazil.
Front Microbiol ; 8: 1642, 2017.
Article em En | MEDLINE | ID: mdl-28932210
Endophytic actinomycetes from medicinal plants produce a wide diversity of secondary metabolites (SM). However, to date, the knowledge about endophytes from Brazil remains scarce. Thus, we analyzed the antimicrobial potential of 10 actinomycetes isolated from the medicinal plant Vochysia divergens located in the Pantanal sul-mato-grossense, an unexplored wetland in Brazil. Strains were classified as belonging to the Aeromicrobium, Actinomadura, Microbacterium, Microbispora, Micrococcus, Sphaerisporangium, Streptomyces, and Williamsia genera, through morphological and 16S rRNA phylogenetic analyzes. A susceptibility analysis demonstrated that the strains were largely resistant to the antibiotics oxacillin and nalidixic acid. Additionally, different culture media (SG and R5A), and temperatures (28 and 36°C) were evaluated to select the best culture conditions to produce the active SM. All conditions were analyzed for active metabolites, and the best antibacterial activity was observed from metabolites produced with SG medium at 36°C. The LGMB491 (close related to Aeromicrobium ponti) extract showed the highest activity against methicillin-resistant Staphylococcus aureus (MRSA), with a MIC of 0.04 mg/mL, and it was selected for SM identification. Strain LGMB491 produced 1-acetyl-ß-carboline (1), indole-3-carbaldehyde (2), 3-(hydroxyacetyl)-indole (4), brevianamide F (5), and cyclo-(L-Pro-L-Phe) (6) as major compounds with antibacterial activity. In this study, we add to the knowledge about the endophytic community from the medicinal plant V. divergens and report the isolation of rare actinomycetes that produce highly active metabolites.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE País/Região como assunto: America do sul / Brasil Idioma: En Revista: Front Microbiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE País/Região como assunto: America do sul / Brasil Idioma: En Revista: Front Microbiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça