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Effect of epithelial growth factor on matrix metalloproteinase-2 and E-cadherin/ß-catenin expression in an in situ model of tumorigenesis.
Navarini, Natalia Festugatto; De Araújo, Vera Cavalcanti; Sperandio, Marcelo; Napimoga, Marcelo Henrique; Teixeira, Lucas Novaes; De Araújo, Ney Soares; Martinez, Elizabeth Ferreira.
Afiliação
  • Navarini NF; Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-755, Brazil.
  • De Araújo VC; Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-755, Brazil.
  • Sperandio M; Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-755, Brazil.
  • Napimoga MH; Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-755, Brazil.
  • Teixeira LN; Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-755, Brazil.
  • De Araújo NS; Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-755, Brazil.
  • Martinez EF; Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-755, Brazil.
Oncol Lett ; 14(3): 3136-3140, 2017 Sep.
Article em En | MEDLINE | ID: mdl-28927057
The aim of the present study was to analyze the in vitro effect of various doses of epidermal growth factor (EGF; 5 and 10 ng/ml) on matrix metalloproteinase-2 (MMP-2) secretion and E-cadherin/ß-catenin expression by co-cultured cells that mimic an in situ carcinoma ex-pleomorphic adenoma, where benign myoepithelial cells from a pleomorphic adenoma surround malignant epithelial cells. EGF was supplemented in various doses and the effects were evaluated following four days of cell culture. ELISA was performed to determine MMP-2 secretion levels. Gene expression for E-cadherin and ß-catenin was analyzed using quantitative polymerase chain reaction. The results revealed that E-cadherin expression decreased when the cells were supplemented with 5 ng/ml EGF. ELISA results indicated that MMP-2 secretion increased when EGF was supplemented at concentrations of 5 and 10 ng/ml. The present findings demonstrated that EGF may be involved in the epithelial-mesenchymal transition process via altering the E-cadherin/ß-catenin complex and increasing MMP-2 secretion, which may then favor the dissolution of the basement membrane to the benefit of malignant cell clusters, contributing to the development of an invasive phenotype in this in vitro model of tumorigenesis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Oncol Lett Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Grécia
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Oncol Lett Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Grécia