Electrochemical Detection of a Dengue-related Oligonucleotide Sequence Using Ferrocenium as a Hybridization Indicator.
Sensors (Basel)
; 7(11): 2510-2518, 2007 Oct 30.
Article
em En
| MEDLINE
| ID: mdl-28903242
A simple method for electrochemical detection of a synthetic 20-bpoligonucleotide sequence related with dengue virus genome was developed. Acomplimentary DNA probe sequence was electrostatically immobilized onto a glassycarbon electrode modified with chitosan. Electrochemical detection of hybridizationbetween probe and target was performed by cyclic voltammetry, using ferrocene (Fc ) as ahybridization label. After hybridization, the peak current response of Fc oxidationincreased around 26%. A higher voltammetric decay rate constant (kd) and a lower half-lifeperiod (t1/2) for the interaction of Fc with dsDNA compared to those with ssDNAquantitatively characterize the different strengths of interaction with both types of DNA.By combining the simplicity of DNA immobilization onto a chitosan film and suitablevoltammetric detection of hybridization concomitant with ferrocene attachment, a gooddiscrimination between ssDNA and dsDNA was obtained.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Sensors (Basel)
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Suíça