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The HIV aspartyl protease inhibitor ritonavir impairs planktonic growth, biofilm formation and proteolytic activity in Trichosporon spp.
Cordeiro, Rossana de Aguiar; Serpa, Rosana; Mendes, Patrícia Bruna Leite; Evangelista, Antonio José de Jesus; Andrade, Ana Raquel Colares; Franco, Jônatas da Silva; Pereira, Vandbergue Dos Santos; Alencar, Lucas Pereira de; Oliveira, Jonathas Sales de; Camargo, Zoilo Pires de; Lima Neto, Reginaldo Gonçalves de; Castelo-Branco, Débora de Souza Collares Maia; Brilhante, Raimunda Samia Nogueira; Rocha, Marcos Fabio Gadelha; Sidrim, José Júlio Costa.
Afiliação
  • Cordeiro RA; a Medical Mycology Specialized Center , Federal University of Ceará , Fortaleza , Brazil.
  • Serpa R; a Medical Mycology Specialized Center , Federal University of Ceará , Fortaleza , Brazil.
  • Mendes PBL; a Medical Mycology Specialized Center , Federal University of Ceará , Fortaleza , Brazil.
  • Evangelista AJJ; a Medical Mycology Specialized Center , Federal University of Ceará , Fortaleza , Brazil.
  • Andrade ARC; a Medical Mycology Specialized Center , Federal University of Ceará , Fortaleza , Brazil.
  • Franco JDS; a Medical Mycology Specialized Center , Federal University of Ceará , Fortaleza , Brazil.
  • Pereira VDS; a Medical Mycology Specialized Center , Federal University of Ceará , Fortaleza , Brazil.
  • Alencar LP; a Medical Mycology Specialized Center , Federal University of Ceará , Fortaleza , Brazil.
  • Oliveira JS; a Medical Mycology Specialized Center , Federal University of Ceará , Fortaleza , Brazil.
  • Camargo ZP; b Department of Microbiology, Immunology and Parasitology , Federal University of São Paulo , São Paulo , Brazil.
  • Lima Neto RG; c Department of Tropical Medicine , Federal University of Pernambuco , Recife , Brazil.
  • Castelo-Branco DSCM; a Medical Mycology Specialized Center , Federal University of Ceará , Fortaleza , Brazil.
  • Brilhante RSN; a Medical Mycology Specialized Center , Federal University of Ceará , Fortaleza , Brazil.
  • Rocha MFG; a Medical Mycology Specialized Center , Federal University of Ceará , Fortaleza , Brazil.
  • Sidrim JJC; d Post Graduate Program in Veterinary Sciences, College of Veterinary Medicine , State University of Ceará , Fortaleza , Brazil.
Biofouling ; 33(8): 640-650, 2017 09.
Article em En | MEDLINE | ID: mdl-28871863
This study evaluated the effect of the protease inhibitor ritonavir (RIT) on Trichosporon asahii and Trichosporon inkin. Susceptibility to RIT was assessed by the broth microdilution assay and the effect of RIT on protease activity was evaluated using azoalbumin as substrate. RIT was tested for its anti-biofilm properties and RIT-treated biofilms were assessed regarding protease activity, ultrastructure and matrix composition. In addition, antifungal susceptibility, surface hydrophobicity and biofilm formation were evaluated after pre-incubation of planktonic cells with RIT for 15 days. RIT (200 µg ml-1) inhibited Trichosporon growth. RIT (100 µg ml-1) also reduced protease activity of planktonic and biofilm cells, decreased cell adhesion and biofilm formation, and altered the structure of the biofilm and the protein composition of the biofilm matrix. Pre-incubation with RIT (100 µg ml-1) increased the susceptibility to amphotericin B, and reduced surface hydrophobicity and cell adhesion. These results highlight the importance of proteases as promising therapeutic targets and reinforce the antifungal potential of protease inhibitors.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plâncton / Trichosporon / Inibidores da Protease de HIV / Biofilmes / Ritonavir / Antifúngicos Idioma: En Revista: Biofouling Assunto da revista: BIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plâncton / Trichosporon / Inibidores da Protease de HIV / Biofilmes / Ritonavir / Antifúngicos Idioma: En Revista: Biofouling Assunto da revista: BIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido