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Do different probing depths exhibit striking differences in microbial profiles?
Pérez-Chaparro, Paula Juliana; McCulloch, John Anthony; Mamizuka, Elsa Masae; Moraes, Aline da Costa Lima; Faveri, Marcelo; Figueiredo, Luciene Cristina; Duarte, Poliana Mendes; Feres, Magda.
Afiliação
  • Pérez-Chaparro PJ; Dental Research Division, Department of Periodontology, Guarulhos University, Guarulhos, Brazil.
  • McCulloch JA; Department of Clinical and Toxicological Analyses, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
  • Mamizuka EM; Trinchieri Lab, Cancer and Inflammation Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
  • Moraes ADCL; Department of Clinical and Toxicological Analyses, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
  • Faveri M; Department of Plant Biology, Biology Institute, State University of Campinas, Campinas, São Paulo, Brazil.
  • Figueiredo LC; Dental Research Division, Department of Periodontology, Guarulhos University, Guarulhos, Brazil.
  • Duarte PM; Dental Research Division, Department of Periodontology, Guarulhos University, Guarulhos, Brazil.
  • Feres M; Dental Research Division, Department of Periodontology, Guarulhos University, Guarulhos, Brazil.
J Clin Periodontol ; 45(1): 26-37, 2018 Jan.
Article em En | MEDLINE | ID: mdl-28871594
AIM: To perform a thorough characterization of the subgingival microbiota of shallow, moderate and deep sites in subjects with chronic periodontitis (ChP). MATERIAL AND METHODS: Subgingival samples were collected from subjects with ChP (n = 3/category of probing depth: ≤3, 4-6 and ≥7 mm) and periodontal health (PH). Individual samples were submitted to 16S rDNA high- throughput sequencing and the analysis was made using mothur and R packages. RESULTS: Nine subjects with ChP and seven with PH were included and 101 samples were evaluated. Thirteen phyla, 118 genera and 211 OTUs were detected. Taxa from Chloroflexi and Spirochaetes phyla were associated with initial stages of disease. Fretibacterium, Eubacterium[XI][G-6], Desulfobulbus, Peptostreptococcaceae[XI][G-1] and [G-3], Bacteroidetes[G-3], Bacteroidaceae[G-1] genera and Filifactor alocis, Fretibacterium fastidiosum, Johnsonella spHOT166, Peptostreptococcaceae[XIII][G-1]HOT113, Porphyromonas endodontalis and Treponema sp. HOT258, which are not conventionally associated with disease, increased with the deepening of the pockets and/or were elevated in ChP; while Streptococcus, Corynebacterium and Bergeyella genera were associated with PH (p < .05). CONCLUSION: Striking differences were observed between the microbiota of shallow and moderate/deep sites, but not between moderate and deep sites in ChP subjects. Differences between shallow sites in PH and ChP were also observed. The characterized microbiota included known oral microorganisms and newly identified periodontal taxa, some of them not-yet-cultured.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Periodontite Crônica / Microbiota Tipo de estudo: Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Adult / Humans / Middle aged Idioma: En Revista: J Clin Periodontol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Periodontite Crônica / Microbiota Tipo de estudo: Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Adult / Humans / Middle aged Idioma: En Revista: J Clin Periodontol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos