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Retrograde trafficking of ß-dystroglycan from the plasma membrane to the nucleus.
Gracida-Jiménez, Viridiana; Mondragón-González, Ricardo; Vélez-Aguilera, Griselda; Vásquez-Limeta, Alejandra; Laredo-Cisneros, Marco S; Gómez-López, Juan de Dios; Vaca, Luis; Gourlay, Sarah C; Jacobs, Laura A; Winder, Steve J; Cisneros, Bulmaro.
Afiliação
  • Gracida-Jiménez V; Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Ciudad de México, Mexico, Mexico.
  • Mondragón-González R; Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Ciudad de México, Mexico, Mexico.
  • Vélez-Aguilera G; Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Ciudad de México, Mexico, Mexico.
  • Vásquez-Limeta A; Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Ciudad de México, Mexico, Mexico.
  • Laredo-Cisneros MS; Laboratory of Protein Dynamics and Signaling, Center for Cancer Research-Frederick, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA.
  • Gómez-López JD; Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Ciudad de México, Mexico, Mexico.
  • Vaca L; Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Ciudad de México, Mexico, Mexico.
  • Gourlay SC; Instituto de Fisiología Celular, Universidad Nacional Autónoma de Mexico, Ciudad de Mexico, Mexico, Mexico.
  • Jacobs LA; Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield, S10 2TN, United Kingdom.
  • Winder SJ; Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield, S10 2TN, United Kingdom.
  • Cisneros B; Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield, S10 2TN, United Kingdom.
Sci Rep ; 7(1): 9906, 2017 08 29.
Article em En | MEDLINE | ID: mdl-28852008
ß-Dystroglycan (ß-DG) is a transmembrane protein with critical roles in cell adhesion, cytoskeleton remodeling and nuclear architecture. This functional diversity is attributed to the ability of ß-DG to target to, and conform specific protein assemblies at the plasma membrane (PM) and nuclear envelope (NE). Although a classical NLS and importin α/ß mediated nuclear import pathway has already been described for ß-DG, the intracellular trafficking route by which ß-DG reaches the nucleus is unknown. In this study, we demonstrated that ß-DG undergoes retrograde intracellular trafficking from the PM to the nucleus via the endosome-ER network. Furthermore, we provided evidence indicating that the translocon complex Sec61 mediates the release of ß-DG from the ER membrane, making it accessible for importins and nuclear import. Finally, we show that phosphorylation of ß-DG at Tyr890 is a key stimulus for ß-DG nuclear translocation. Collectively our data describe the retrograde intracellular trafficking route that ß-DG follows from PM to the nucleus. This dual role for a cell adhesion receptor permits the cell to functionally connect the PM with the nucleus and represents to our knowledge the first example of a cell adhesion receptor exhibiting retrograde nuclear trafficking and having dual roles in PM and NE.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Sci Rep Ano de publicação: 2017 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Sci Rep Ano de publicação: 2017 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido