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Notable fibrolytic enzyme production by Aspergillus spp. isolates from the gastrointestinal tract of beef cattle fed in lignified pastures.
Abrão, Flávia Oliveira; Duarte, Eduardo Robson; Pessoa, Moisés Sena; Santos, Vera Lúcia Dos; Freitas Júnior, Luiz Fernando de; Barros, Katharina de Oliveira; Hughes, Alice Ferreira da Silva; Silva, Thiago Dias; Rodriguez, Norberto Mário.
Afiliação
  • Abrão FO; Instituto Federal Goiano, Ceres, Brasil.
  • Duarte ER; Instituto de Ciências Agrárias da Universidade Federal de Minas Gerais (UFMG), Montes Claros, Brasil.
  • Pessoa MS; Universidade Federal de Goiás, Goiânia, Brasil.
  • Santos VLD; Instituto de Ciências Biológicas da UFMG, Belo Horizonte, Brasil.
  • Freitas Júnior LF; Instituto de Ciências Biológicas da UFMG, Belo Horizonte, Brasil.
  • Barros KO; Instituto de Ciências Biológicas da UFMG, Belo Horizonte, Brasil.
  • Hughes AFDS; Instituto de Ciências Biológicas da UFMG, Belo Horizonte, Brasil.
  • Silva TD; Instituto Federal Goiano, Ceres, Brasil.
  • Rodriguez NM; Escola de Veterinária da UFMG, Belo Horizonte, Brasil.
PLoS One ; 12(8): e0183628, 2017.
Article em En | MEDLINE | ID: mdl-28850605
Fungi have the ability to degrade vegetal cell wall carbohydrates, and their presence in the digestive tract of ruminants can minimize the effects of lignified forage on ruminal fermentation. Here, we evaluated enzyme production by Aspergillus spp. isolates from the digestive tracts of cattle grazed in tropical pastures during the dry season. Filamentous fungi were isolated from rumen and feces by culture in cellulose-based medium. Ninety fungal strains were isolated and identified by rDNA sequence analysis, microculture, or both. Aspergillus terreus was the most frequently isolated species, followed by Aspergillus fumigatus. The isolates were characterized with respect to their cellulolytic, xylanolytic, and lignolytic activity through qualitative evaluation in culture medium containing a specific corresponding carbon source. Carboxymethyl cellulase (CMCase) activity was quantified by the reducing sugar method. In the avicel and xilan degradation test, the enzyme activity (EA) at 48 h was significantly higher other periods (P < 0.05). Intra- and inter-specific differences in EA were verified, and high levels of phenoloxidases, which are crucial for lignin degradation, were observed in 28.9% of the isolates. Aspergillus terreus showed significantly higher EA for avicelase (3.96 ±1.77) and xylanase (3.13 ±.091) than the other Aspergillus species at 48 h of incubation. Isolates AT13 and AF69 showed the highest CMCase specific activity (54.84 and 33.03 U mg-1 protein, respectively). Selected Aspergillus spp. isolates produced remarkable levels of enzymes involved in vegetal cell wall degradation, suggesting their potential as antimicrobial additives or probiotics in ruminant diets.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aspergillus / Celulase / Trato Gastrointestinal / Celulases / Endo-1,4-beta-Xilanases / Lignina Tipo de estudo: Qualitative_research Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aspergillus / Celulase / Trato Gastrointestinal / Celulases / Endo-1,4-beta-Xilanases / Lignina Tipo de estudo: Qualitative_research Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos