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A comparison between a new vitrification protocol and the slow freezing method in the cryopreservation of prepubertal testicular tissue.
Radaelli, Moacir R M; Almodin, Carlos G; Minguetti-Câmara, Vânia C; Cerialli, Paula Motta Almodin; Nassif, Aissar E; Gonçalves, Antonio J.
Afiliação
  • Radaelli MRM; Urology Department, Medical School, Faculdade Ingá, Maringá, Brazil.
  • Almodin CG; Surgery Department, Santa Casa de São Paulo Medical Sciences School, São Paulo, Brazil.
  • Minguetti-Câmara VC; Materbaby - Human Reproduction and Genetics Clinic, Maringá - 87.013-230, Brazil.
  • Cerialli PMA; Materbaby - Human Reproduction and Genetics Clinic, Maringá - 87.013-230, Brazil.
  • Nassif AE; Materbaby - Human Reproduction and Genetics Clinic, Maringá - 87.013-230, Brazil.
  • Gonçalves AJ; Materbaby - Human Reproduction and Genetics Clinic, Maringá - 87.013-230, Brazil.
JBRA Assist Reprod ; 21(3): 188-195, 2017 09 01.
Article em En | MEDLINE | ID: mdl-28837026
OBJECTIVE: This study aimed to compare a new vitrification protocol with reduced cryoprotectant exposure to the slow freezing method in the cryopreservation of prepubertal rat testicular tissue. METHODS: Five sexually immature male Wistar rats were submitted to bilateral orchiectomy. Tissue samples from each testicle were fragmented into small pieces and randomly assigned to three groups: Group A, fresh tissue (control); Group B, slow programmable freezing (SPF); and Group C (vitrification). Frozen/thawed, vitrified/warmed, and fresh testicular tissue were histologically compared. A pathologist blinded to the procedures assessed the morphology (cell differentiation, nuclei, and epithelium) of 10 seminiferous tubules from each testicle (100 tubules per Group). RESULTS: Sertoli and spermatogonial stem cells were easily differentiated, and the nucleoli were easily viewed in the tubules assessed in all three groups. Small alterations in tissue architecture were observed in the control group as a result of tissue handling. Moderate alterations of the epithelium with the formation of small gaps and cell detachment from the basement membrane were observed in 28% of the frozen and 9% of the vitrified tubules. Condensed nuclei involving a small proportion of cells were observed in six and three tubules of the frozen and vitrified group, respectively. Despite the alterations, 97% of the frozen and 99% of the vitrified tubules were considered well preserved. CONCLUSIONS: The findings indicate that the vitrification protocol tested in this study adequately preserved the morphological integrity of prepubertal testicular tissue in a rat model. Further studies are required to confirm testicular tissue function after grafting.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Testículo / Criopreservação / Vitrificação / Congelamento Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: JBRA Assist Reprod Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Testículo / Criopreservação / Vitrificação / Congelamento Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: JBRA Assist Reprod Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil