Your browser doesn't support javascript.
loading
Extraction, purification and structural characterization of a galactoglucomannan from the gabiroba fruit (Campomanesia xanthocarpa Berg), Myrtaceae family.
Barbieri, Shayla Fernanda; Ruthes, Andrea Caroline; Petkowicz, Carmen Lúcia de Oliveira; de Godoy, Rossana Catie Bueno; Sassaki, Guilherme Lanzi; Santana Filho, Arquimedes Paixão; Silveira, Joana Léa Meira.
Afiliação
  • Barbieri SF; Biochemistry and Molecular Biology Department, Federal University of Paraná, CEP 81.531-980, Curitiba, PR, Brazil.
  • Ruthes AC; Division of Glycoscience, Royal Institute of Technology - KTH, Sweden.
  • Petkowicz CLO; Biochemistry and Molecular Biology Department, Federal University of Paraná, CEP 81.531-980, Curitiba, PR, Brazil.
  • de Godoy RCB; Brazilian Agricultural Research Corporation, Embrapa Forestry, CEP 83.411-000, Colombo PR, Brazil.
  • Sassaki GL; Biochemistry and Molecular Biology Department, Federal University of Paraná, CEP 81.531-980, Curitiba, PR, Brazil.
  • Santana Filho AP; Biochemistry and Molecular Biology Department, Federal University of Paraná, CEP 81.531-980, Curitiba, PR, Brazil.
  • Silveira JLM; Biochemistry and Molecular Biology Department, Federal University of Paraná, CEP 81.531-980, Curitiba, PR, Brazil. Electronic address: jlms12@yahoo.com.
Carbohydr Polym ; 174: 887-895, 2017 Oct 15.
Article em En | MEDLINE | ID: mdl-28821144
In this study, we isolated and structurally characterized, for the first time, a galactoglucomannan (GGM) from the pulp of gabiroba, a Myrtaceae family species. The HPSEC-MALLS-RI analysis showed a homogeneous polysaccharide with molar mass of 25,340gmol-1. The monosaccharide composition showed that the GGM consisted of Man:Glc:Gal in a molar ratio of 1:1:0.6. Methylation and 1D and 2D NMR analyses suggested that the main chain of the GGM consisted of ß-d-Glcp and ß-d-Manp units (1→4)-linked. The α-d-Galp substitutions occur mainly at O-6 position of ß-d-Manp units. The glycosidic linkages of the GGM were evident by the presence of the characteristic signals of 4-O-substituted residues at δ 78.6/3.69 for both ß-d-Glcp and ß-d-Manp. Furthermore, the O-6 substitutions for both ß-d-Glcp and ß-d-Manp units were confirmed by signals at δ 67.1/4.00 and 3.93. The interglycosidic correlations, obtained through the analysis of the HMBC spectrum, further confirm the structure.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Myrtaceae / Frutas / Mananas Idioma: En Revista: Carbohydr Polym Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Myrtaceae / Frutas / Mananas Idioma: En Revista: Carbohydr Polym Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido