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Carbon Nanotubes Arranged As Smart Interfaces in Lipid Langmuir-Blodgett Films Enhancing the Enzymatic Properties of Penicillinase for Biosensing Applications.
Scholl, Fabio A; Morais, Paulo V; Gabriel, Rayla C; Schöning, Michael J; Siqueira, José R; Caseli, Luciano.
Afiliação
  • Scholl FA; Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo (UNIFESP) , 09913-030 Diadema, São Paulo, Brazil.
  • Morais PV; Institute of Exact Sciences, Natural and Education, Federal University of Triângulo Mineiro (UFTM) , 38064-200 Uberaba, Minas Gerais, Brazil.
  • Gabriel RC; Institute of Exact Sciences, Natural and Education, Federal University of Triângulo Mineiro (UFTM) , 38064-200 Uberaba, Minas Gerais, Brazil.
  • Schöning MJ; Institute of Nano- and Biotechnologies (INB) , FH Aachen, Campus Jülich, 52428 Jülich, Germany.
  • Siqueira JR; Peter Grünberg Institute (PGI-8) , Forschungszentrum Jülich, 52425 Jülich, Germany.
  • Caseli L; Institute of Exact Sciences, Natural and Education, Federal University of Triângulo Mineiro (UFTM) , 38064-200 Uberaba, Minas Gerais, Brazil.
ACS Appl Mater Interfaces ; 9(36): 31054-31066, 2017 Sep 13.
Article em En | MEDLINE | ID: mdl-28816431
In this paper, carbon nanotubes (CNTs) were incorporated in penicillinase-phospholipid Langmuir and Langmuir-Blodgett (LB) films to enhance the enzyme catalytic properties. Adsorption of the penicillinase and CNTs at dimyristoylphosphatidic acid (DMPA) monolayers at the air-water interface was investigated by surface pressure-area isotherms, vibrational spectroscopy, and Brewster angle microscopy. The floating monolayers were transferred to solid supports through the LB technique, forming mixed DMPA-CNTs-PEN films, which were investigated by quartz crystal microbalance, vibrational spectroscopy, and atomic force microscopy. Enzyme activity was studied with UV-vis spectroscopy and the feasibility of the supramolecular device nanostructured as ultrathin films were essayed in a capacitive electrolyte-insulator-semiconductor (EIS) sensor device. The presence of CNTs in the enzyme-lipid LB film not only tuned the catalytic activity of penicillinase but also helped conserve its enzyme activity after weeks, showing increased values of activity. Viability as penicillin sensor was demonstrated with capacitance/voltage and constant capacitance measurements, exhibiting regular and distinctive output signals over all concentrations used in this work. These results may be related not only to the nanostructured system provided by the film, but also to the synergism between the compounds on the active layer, leading to a surface morphology that allowed a fast analyte diffusion because of an adequate molecular accommodation, which also preserved the penicillinase activity. This work therefore demonstrates the feasibility of employing LB films composed of lipids, CNTs, and enzymes as EIS devices for biosensing applications.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanotubos de Carbono Idioma: En Revista: ACS Appl Mater Interfaces Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanotubos de Carbono Idioma: En Revista: ACS Appl Mater Interfaces Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos