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Conorfamide-Sr3, a structurally novel specific inhibitor of the Shaker K+ channel.
Campos-Lira, Elba; Carrillo, Elisa; Aguilar, Manuel B; Gajewiak, Joanna; Gómez-Lagunas, Froylán; López-Vera, Estuardo.
Afiliação
  • Campos-Lira E; Laboratorio de Toxinología Marina, Unidad Académica de Ecología y Biodiversidad Acuática, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México, 04510, Mexico.
  • Carrillo E; Laboratorio de Toxinología Marina, Unidad Académica de Ecología y Biodiversidad Acuática, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México, 04510, Mexico.
  • Aguilar MB; Laboratorio de Neurofarmacología Marina, Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Campus Juriquilla, Querétaro, 76230, Mexico.
  • Gajewiak J; Department of Biology, University of Utah, 257 South 1400 East, Salt Lake City, UT, 84112, USA.
  • Gómez-Lagunas F; Department of Physiology, School of Medicine, National Autonomous University of México, Ciudad Universitaria, México City, 04510, Mexico. Electronic address: froylangl@yahoo.com.
  • López-Vera E; Laboratorio de Toxinología Marina, Unidad Académica de Ecología y Biodiversidad Acuática, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México, 04510, Mexico. Electronic address: vera@cmarl.unam.mx.
Toxicon ; 138: 53-58, 2017 Nov.
Article em En | MEDLINE | ID: mdl-28774677
Conorfamides (CNFs) are toxins initially characterized from the venom duct of the venomous marine snail Conus spurius from the Gulf of Mexico; at their C-termini, these toxins are amidated and have high sequence similarity with the molluskan cardioexcitatory tetrapeptide Phe-Met-Arg-Phe-NH2 (FMRFamide or FMRFa) and other FMRFa-related peptides (FaRPs) found in the five molluskan classes, and in other invertebrate and vertebrate phyla. These peptides were the first FaRPs found to be present in any venom, and they are biologically active in mice, limpets, and/or freshwater snails. However, the molecular targets of the known CNFs (CNF-Sr1 and CNF-Sr2 from C. spurius, and CNF-Vc1 from C. victoriae) remain unidentified. Very recently, three FaRPs from C. textile have been found to potentiate the currents of acid-sensing ion channels. In this work, we characterized a novel conorfamide, CNF-Sr3 (ATSGPMGWLPVFYRF-NH2), comprised of 15 amino acid residues, and with a specific blocking activity for the Shaker subtype of the voltage-gated potassium channels, without significant effect on the Shab, Shaw, Shal and Eag channels. This peptide is the third type of disulfide-free conotoxins that has been discovered to target K+ channels.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Neuropeptídeos / Caramujo Conus / Superfamília Shaker de Canais de Potássio / Venenos de Moluscos Limite: Animals / Humans Idioma: En Revista: Toxicon Ano de publicação: 2017 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Neuropeptídeos / Caramujo Conus / Superfamília Shaker de Canais de Potássio / Venenos de Moluscos Limite: Animals / Humans Idioma: En Revista: Toxicon Ano de publicação: 2017 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido