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Osteoblast Cell Response on the Ti6Al4V Alloy Heat-Treated.
Chávez-Díaz, Mercedes Paulina; Escudero-Rincón, María Lorenza; Arce-Estrada, Elsa Miriam; Cabrera-Sierra, Román.
Afiliação
  • Chávez-Díaz MP; Departamento de Ingeniería en Metalurgia y Materiales, Instituto Politécnico Nacional (ESIQIE-IPN), UPALM Zacatenco, Ciudad de México 07738, Mexico. mpaulinachavezdiaz@yahoo.com.
  • Escudero-Rincón ML; Departamento de Ingeniería de Superficies, Corrosión y Durabilidad, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Madrid 28040, Spain. mpaulinachavezdiaz@yahoo.com.
  • Arce-Estrada EM; Departamento de Ingeniería de Superficies, Corrosión y Durabilidad, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Madrid 28040, Spain. escudero@cenim.csic.es.
  • Cabrera-Sierra R; Departamento de Ingeniería en Metalurgia y Materiales, Instituto Politécnico Nacional (ESIQIE-IPN), UPALM Zacatenco, Ciudad de México 07738, Mexico. earce@ipn.mx.
Materials (Basel) ; 10(4)2017 Apr 23.
Article em En | MEDLINE | ID: mdl-28772804
In an effort to examine the effect of the microstructural changes of the Ti6Al4V alloy, two heat treatments were carried out below (Ti6Al4V800) and above (Ti6Al4V1050) its ß-phase transformation temperature. After each treatment, globular and lamellar microstructures were obtained. Saos-2 pre-osteoblast human osteosarcoma cells were seeded onto Ti6Al4V alloy disks and immersed in cell culture for 7 days. Electrochemical assays in situ were performed using OCP and EIS measurements. Impedance data show a passive behavior for the three Ti6Al4V alloys; additionally, enhanced impedance values were recorded for Ti6Al4V800 and Ti6Al4V1050 alloys. This passive behavior in culture medium is mostly due to the formation of TiO2 during their sterilization. Biocompatibility and cell adhesion were characterized using the SEM technique; Ti6Al4V as received and Ti6Al4V800 alloys exhibited polygonal and elongated morphology, whereas Ti6Al4V1050 alloy displayed a spherical morphology. Ti and O elements were identified by EDX analysis due to the TiO2 and signals of C, N and O, related to the formation of organic compounds from extracellular matrix. These results suggest that cell adhesion is more likely to occur on TiO2 formed in discrete α-phase regions (hcp) depending on its microstructure (grains).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Materials (Basel) Ano de publicação: 2017 Tipo de documento: Article País de afiliação: México País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Materials (Basel) Ano de publicação: 2017 Tipo de documento: Article País de afiliação: México País de publicação: Suíça