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Genetic analysis of a PER-2-producing Shewanella sp. strain harbouring a variety of mobile genetic elements and antibiotic resistance determinants.
Almuzara, Marisa; Montaña, Sabrina; Lazzaro, Terese; Uong, Sylvia; Parmeciano Di Noto, Gisela; Traglia, German; Bakai, Romina; Centrón, Daniela; Iriarte, Andrés; Quiroga, Cecilia; Ramirez, Maria Soledad.
Afiliação
  • Almuzara M; Laboratorio de Bacteriología, Hospital Interzonal de Agudos Eva Perón, San Martín, Buenos Aires, Argentina.
  • Montaña S; Instituto de Microbiología (IMPaM), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Lazzaro T; Department of Biological Science, California State University, Fullerton, 800 N. State College Blvd., Fullerton, CA 92831, USA.
  • Uong S; Department of Biological Science, California State University, Fullerton, 800 N. State College Blvd., Fullerton, CA 92831, USA.
  • Parmeciano Di Noto G; Instituto de Microbiología (IMPaM), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Traglia G; Instituto de Microbiología (IMPaM), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Bakai R; Laboratorio de Bacteriología, Hospital Interzonal de Agudos Eva Perón, San Martín, Buenos Aires, Argentina.
  • Centrón D; Instituto de Microbiología (IMPaM), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Iriarte A; Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, UdelaR, Montevideo, Uruguay.
  • Quiroga C; Instituto de Microbiología (IMPaM), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Ramirez MS; Department of Biological Science, California State University, Fullerton, 800 N. State College Blvd., Fullerton, CA 92831, USA. Electronic address: msramirez@fullerton.edu.
J Glob Antimicrob Resist ; 11: 81-86, 2017 12.
Article em En | MEDLINE | ID: mdl-28760680
The objective of this study was to investigate the molecular mechanisms explaining the multidrug-resistant (MDR) phenotype found in a novel clinical Shewanella sp. strain (Shew256) recovered from a diabetic patient. Whole-genome shotgun sequencing was performed using Illumina MiSeq-I and Nextera XT DNA library. De novo assembly was performed with SPAdes. RAST Server was used to predict the open-reading frames and the predictions were confirmed using BLAST. Further genomic analysis was carried out using average nucleotide identity (ANI), ACT (Artemis), OrthoMCL, ARG-ANNOT, ISfinder, PHAST, tRNAscan-SE, plasmidSPAdes, PlasmidFinder and MAUVE. PCR and plasmid extraction were also performed. Genomic analysis revealed a total of 456 predicted genes unique to Shew256 compared with other Shewanella genomes. Moreover, the presence of different resistance genes, including blaPER-2, was found. A complex class 1 integron containing the ISCR1 gene, disrupted by two putative transposase genes, was identified. Furthermore, other resistance genes, a transposon containing aph(3'), insertion sequences, phages and non-coding RNAs were also found. In conclusion, evidence of acquisition of resistance genes and mobile elements that could explain the MDR phenotype were observed. This Shewanella sp. represents a prime example of how antibiotic resistance determinants can be acquired by uncommon pathogens.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Beta-Lactamases / Elementos de DNA Transponíveis / Shewanella / Farmacorresistência Bacteriana Múltipla / Genes Bacterianos Tipo de estudo: Prognostic_studies Limite: Aged / Humans / Male Idioma: En Revista: J Glob Antimicrob Resist Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Argentina País de publicação: Holanda
Texto completo
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Beta-Lactamases / Elementos de DNA Transponíveis / Shewanella / Farmacorresistência Bacteriana Múltipla / Genes Bacterianos Tipo de estudo: Prognostic_studies Limite: Aged / Humans / Male Idioma: En Revista: J Glob Antimicrob Resist Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Argentina País de publicação: Holanda