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Sensitivity of Enzymatic Toxins from Corneal Isolate of Acanthamoeba Protozoan to Physicochemical Parameters.
Sant'Ana, Viviane P; Foronda, Annette S; de Freitas, Denise; Carrijo-Carvalho, Linda C; de Souza Carvalho, Fábio Ramos.
Afiliação
  • Sant'Ana VP; Department of Ophthalmology and Visual Sciences, Paulista School of Medicine, Federal University of Sao Paulo, Botucatu Street, 821, Vila Clementino, Sao Paulo, SP, 04023-062, Brazil.
  • Foronda AS; Department of Ophthalmology and Visual Sciences, Paulista School of Medicine, Federal University of Sao Paulo, Botucatu Street, 821, Vila Clementino, Sao Paulo, SP, 04023-062, Brazil.
  • de Freitas D; Department of Ophthalmology and Visual Sciences, Paulista School of Medicine, Federal University of Sao Paulo, Botucatu Street, 821, Vila Clementino, Sao Paulo, SP, 04023-062, Brazil.
  • Carrijo-Carvalho LC; Department of Ophthalmology and Visual Sciences, Paulista School of Medicine, Federal University of Sao Paulo, Botucatu Street, 821, Vila Clementino, Sao Paulo, SP, 04023-062, Brazil.
  • de Souza Carvalho FR; Centro Universitário do Espírito Santo (UNESC), Colatina, Espirito Santo, Brazil.
Curr Microbiol ; 74(11): 1316-1323, 2017 Nov.
Article em En | MEDLINE | ID: mdl-28755026
Acanthamoeba is a free-living amoeba that causes severe corneal infection (Acanthamoeba keratitis) and produces a variety of extracellular enzymes, called exoproteome. Since physicochemical characters are suggested being associated with therapeutic profile and clinical severity of the infection, we investigated the physicochemical properties of proteolysis mediated by amoebic exoproteome. Corneal scraping was collected from a patient who showed typical symptoms of acute Acanthamoeba keratitis. Axenic amoeba was phylogenetically identified by 18S rDNA sequencing analysis. Effects of pH, temperature and diamidines on proteolysis mediated by exoproteome were assessed using zymography assays. Proteolytic enzymes were most active at pH 7.0 and 37 °C. Calcium ions decreased enzymatic activity. The main components of amoebic exoproteome were characterized as serine proteases. We demonstrated for the first time that commercial antimicrobial diamidines used for Acanthamoeba keratitis therapy inhibit enzymatic activity of amoebic exoproteome. Results showed the thermostability of Acanthamoeba proteases, which suggest a long-term effect of these virulence factors at the central and peripheral cornea with possible role in degradation of extracellular matrix components. Our findings open new perspectives about the complementary and unreported properties of antimicrobial compounds of the diamidine class on the inhibition of enzymatic activity and presumptive control of amoebic infection in the cornea.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Toxinas Biológicas / Acanthamoeba / Córnea Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Adult / Female / Humans Idioma: En Revista: Curr Microbiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Toxinas Biológicas / Acanthamoeba / Córnea Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Adult / Female / Humans Idioma: En Revista: Curr Microbiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos