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Caspase-3 gene expression in human luteinized granulosa cells is inversely correlated with the number of oocytes retrieved after controlled ovarian stimulation.
Lobach, Verônica N; Casalechi, Maíra; Dela Cruz, Cynthia; Pereira, Maria T; Del Puerto, Helen L; Reis, Fernando M.
Afiliação
  • Lobach VN; a Division of Human Reproduction, Department of Obstetrics and Gynecology , Universidade Federal de Minas Gerais , Belo Horizonte , Brazil.
  • Casalechi M; a Division of Human Reproduction, Department of Obstetrics and Gynecology , Universidade Federal de Minas Gerais , Belo Horizonte , Brazil.
  • Dela Cruz C; a Division of Human Reproduction, Department of Obstetrics and Gynecology , Universidade Federal de Minas Gerais , Belo Horizonte , Brazil.
  • Pereira MT; a Division of Human Reproduction, Department of Obstetrics and Gynecology , Universidade Federal de Minas Gerais , Belo Horizonte , Brazil.
  • Del Puerto HL; b Department of Pathology , Universidade Federal de Minas Gerais , Belo Horizonte , Brazil.
  • Reis FM; a Division of Human Reproduction, Department of Obstetrics and Gynecology , Universidade Federal de Minas Gerais , Belo Horizonte , Brazil.
Hum Fertil (Camb) ; 22(1): 33-38, 2019 Apr.
Article em En | MEDLINE | ID: mdl-28738699
Granulosa cells control oocyte maturation through paracrine signalling and changes to the microenvironment around the oocyte. Apoptosis occurs as a physiological mechanism of granulosa cell renewal, but how it relates with the ovarian response to induced ovulation is still unclear. Therefore, this study evaluated apoptosis-related gene expression levels in granulosa cells of patients undergoing controlled ovarian stimulation. We enrolled prospectively 59 consecutive IVF patients referred to a tertiary academic hospital for couple infertility treatment. Luteinized granulosa cells were isolated from follicular fluid and the RNA was extracted, reverse-transcribed and the gene expression of apoptosis inducers (caspase-3, caspase-8 and bax) and inhibitor (Bcl-2) was quantified by real-time polymerase chain reaction. Caspase-3 gene expression correlated negatively with the number of pre-ovulatory follicles (Spearman's r = -0.308), the number of collected oocytes (r = -0.451), the number of mature oocytes (r = -0.526), the number of fertilized oocytes (r = -0.439) and the number of viable embryos (r = -0.443, all statistically significant at p < 0.02 level). No such associations were found with caspase-8, bax or bcl-2. These preliminary findings suggest that increased caspase-3 gene expression in granulosa cells is associated with a worse ovulatory response in humans.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Indução da Ovulação / Hormônio Liberador de Gonadotropina / Nafarelina / Caspase 3 / Células da Granulosa Tipo de estudo: Etiology_studies / Incidence_studies / Observational_studies / Risk_factors_studies Limite: Female / Humans Idioma: En Revista: Hum Fertil (Camb) Assunto da revista: MEDICINA REPRODUTIVA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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XML
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Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Indução da Ovulação / Hormônio Liberador de Gonadotropina / Nafarelina / Caspase 3 / Células da Granulosa Tipo de estudo: Etiology_studies / Incidence_studies / Observational_studies / Risk_factors_studies Limite: Female / Humans Idioma: En Revista: Hum Fertil (Camb) Assunto da revista: MEDICINA REPRODUTIVA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido