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Selection of Biodegrading Phytosterol Strains.
Mondaca, María-Angélica; Vidal, Maricel; Chamorro, Soledad; Vidal, Gladys.
Afiliação
  • Mondaca MA; Microbiology Department, University of Concepción, P.O. Box 160-C, Concepción, Chile.
  • Vidal M; Microbiology Department, University of Concepción, P.O. Box 160-C, Concepción, Chile.
  • Chamorro S; Engineering and Environmental Biotechnology Group, Environmental Science Faculty & Center EULA-Chile, University of Concepción, P.O. Box 160-C, Concepción, Chile.
  • Vidal G; Engineering and Environmental Biotechnology Group, Environmental Science Faculty & Center EULA-Chile, University of Concepción, P.O. Box 160-C, Concepción, Chile. glvidal@udec.cl.
Methods Mol Biol ; 1645: 143-150, 2017.
Article em En | MEDLINE | ID: mdl-28710625
The phytosterol-biotransforming strains were selected from Mycobacterium sp., using a high concentration of ß-sitosterol. The selection was made by culturing the strains in a medium enriched with 14 g ß-sitosterol/L as the unique source of carbon. During 2 months, the bacterial cultures were transferred successively. The extraction of the biotransformation products was made with methanol and ethyl acetate. The qualitative and quantitative analysis was made by means of thin-layer chromatography, gas-liquid chromatography (GLC), and GLC-mass spectrometry. Under these conditions, it was observed that after seven transfers, the strains Mycobacterium sp. MB-3683 and the Mycobacterium fortuitum B-11045 increased their biotransformation capacity from 20% to 64% and from 34% to 55%, respectively. The products in the highest proportion identified for each trial were androstenedione and androstadienedione. The results suggest that the high substrate concentration could be a selective mechanism to obtain strains more efficient in the biotransformation of ß-sitosterol into steroidal bases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fitosteróis / Sitosteroides / Biotransformação / Cromatografia Gasosa-Espectrometria de Massas Tipo de estudo: Qualitative_research Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fitosteróis / Sitosteroides / Biotransformação / Cromatografia Gasosa-Espectrometria de Massas Tipo de estudo: Qualitative_research Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos