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Chemical composition and cytotoxicity of extracts of marjoram and rosemary and their activity against Sporothrix brasiliensis.
Waller, Stefanie Bressan; Madrid, Isabel Martins; Hoffmann, Jéssica Fernanda; Picoli, Tony; Cleff, Marlete Brum; Chaves, Fábio Clasen; Faria, Renata Osório de; Meireles, Mário Carlos Araújo; Braga de Mello, João Roberto.
Afiliação
  • Waller SB; Department of Preventive Veterinary, College of Veterinary, Federal University of Pelotas, Pelotas/RS, Brazil.
  • Madrid IM; Center for Zoonosis Control, City Hall of Pelotas, Pelotas/RS, Brazil.
  • Hoffmann JF; Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas/RS, Brazil.
  • Picoli T; Department of Preventive Veterinary, College of Veterinary, Federal University of Pelotas, Pelotas/RS, Brazil.
  • Cleff MB; Department of Preventive Veterinary, College of Veterinary, Federal University of Pelotas, Pelotas/RS, Brazil.
  • Chaves FC; Department of Agroindustrial Science and Technology, Federal University of Pelotas, Pelotas/RS, Brazil.
  • Faria RO; Department of Preventive Veterinary, College of Veterinary, Federal University of Pelotas, Pelotas/RS, Brazil.
  • Meireles MCA; Department of Preventive Veterinary, College of Veterinary, Federal University of Pelotas, Pelotas/RS, Brazil.
  • Braga de Mello JR; Department of Pharmacology, Institute of Basic Sciences and Health, Federal University of Rio Grande do Sul, Porto Alegre/RS, Brazil.
J Med Microbiol ; 66(7): 1076-1083, 2017 Jul.
Article em En | MEDLINE | ID: mdl-28699881
PURPOSE: Motivated by increasing reports of antifungal resistance in human and animal sporotrichosis, this study evaluated the chemical composition, cytotoxicity and anti-Sporothrix brasiliensis activity of extracts of marjoram (Origanum majorana) and rosemary (Rosmarinus officinalis). METHODOLOGY: Ten (INF10) and 60 min (INF60) infusions, a decoction and a hydroalcoholic extract (HAE, 70 %) were prepared from both plants (10 % w/v). The extract composition was analysed by liquid chromatography/mass spectrometry and the cytotoxicity was evaluated using a colorimetric assay in canine and feline kidney cells. Using a broth microdilution assay (CLSI M38-A2) adapted to the extracts, 30 Sporothrix brasiliensis isolates from dogs, cats and humans, and one Sporothrix schenckii were tested.Results/Key findings. The predominant phenolic compounds found in all extracts were 4-hydroxybenzoic acid, caffeic acid and chlorogenic acid. Luteolin was also one of the predominant compounds, but only in the HAE of marjoram. Extracts of marjoram maintained cell viability in concentrations up to 2.5 mg ml-1 for the feline cell line and up to 10 mg ml-1 for the canine cell line, whereas in rosemary, the cell viability for both kidney lines was maintained with concentrations up to 5 mg ml-1. The activity of rosemary extracts was low or absent. Among the marjoram extracts, HAE was highlighted and had fungistatic activity against Sporothrix brasiliensis (MIC5040 mg ml-1), including in all itraconazole-resistant isolates. S. schenckiisensu stricto was sensitive to marjoram extracts (MIC/MFC ≤5 mg ml-1), with the exception of INF10. CONCLUSION: These findings support the potential usefulness of the HAE of marjoram in the treatment of sporotrichosis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sporothrix / Extratos Vegetais / Rosmarinus / Origanum / Compostos Fitoquímicos / Antifúngicos Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Med Microbiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sporothrix / Extratos Vegetais / Rosmarinus / Origanum / Compostos Fitoquímicos / Antifúngicos Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Med Microbiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido