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The flavonoid rutin modulates microglial/macrophage activation to a CD150/CD206 M2 phenotype.
Bispo da Silva, Alessandra; Cerqueira Coelho, Paulo Lucas; Alves Oliveira Amparo, Jessika; Alves de Almeida Carneiro, Monique Marylin; Pereira Borges, Julita Maria; Dos Santos Souza, Cleide; Dias Costa, Maria de Fátima; Mecha, Miriam; Guaza Rodriguez, Carmen; Amaral da Silva, Victor Diogenes; Lima Costa, Silvia.
Afiliação
  • Bispo da Silva A; Laboratory of Neurochemistry and Cell Biology, Department of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, 40110-100, Salvador, Bahia, Brazil.
  • Cerqueira Coelho PL; Laboratory of Neurochemistry and Cell Biology, Department of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, 40110-100, Salvador, Bahia, Brazil.
  • Alves Oliveira Amparo J; Laboratory of Neurochemistry and Cell Biology, Department of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, 40110-100, Salvador, Bahia, Brazil.
  • Alves de Almeida Carneiro MM; Laboratory of Neurochemistry and Cell Biology, Department of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, 40110-100, Salvador, Bahia, Brazil.
  • Pereira Borges JM; Laboratory of Neurochemistry and Cell Biology, Department of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, 40110-100, Salvador, Bahia, Brazil.
  • Dos Santos Souza C; Laboratory of Neurochemistry and Cell Biology, Department of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, 40110-100, Salvador, Bahia, Brazil.
  • Dias Costa MF; Laboratory of Neurochemistry and Cell Biology, Department of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, 40110-100, Salvador, Bahia, Brazil.
  • Mecha M; Neuroimmunology Group, Department of Functional and System Neurobiology, Instituto Cajal, Consejo Superior de Investigaciones Científicas, Avenida Doctor Arce 37, 28002 Madrid, Spain.
  • Guaza Rodriguez C; Neuroimmunology Group, Department of Functional and System Neurobiology, Instituto Cajal, Consejo Superior de Investigaciones Científicas, Avenida Doctor Arce 37, 28002 Madrid, Spain.
  • Amaral da Silva VD; Laboratory of Neurochemistry and Cell Biology, Department of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, 40110-100, Salvador, Bahia, Brazil.
  • Lima Costa S; Laboratory of Neurochemistry and Cell Biology, Department of Biochemistry and Biophysics, Institute of Health Sciences, Federal University of Bahia, 40110-100, Salvador, Bahia, Brazil. Electronic address: costasl@ufba.br.
Chem Biol Interact ; 274: 89-99, 2017 Aug 25.
Article em En | MEDLINE | ID: mdl-28693884
Rutin is a glycosylated flavonoid present in many fruits and plants that has been demonstrated to have anti-inflammatory and antioxidant properties. However, little is known about the mechanisms underlying microglial activation and its effects on the regulation of cytokines and chemokines associated with inflammatory responses in the central nervous system. In this study we examined the effect of rutin on resting or lipopolysaccharide (LPS)-stimulated microglia and characterized their modulation to an activated M1 phenotype or an alternatively activated M2 phenotype. Microglial cells were treated with rutin (1-100 µM); alternatively, microglial cells were stimulated with LPS and the cells were then treated with rutin (50 µM). The results revealed that rutin treatment was not toxic to microglial cells and induced a dose-dependent increase in microglial proliferation associated with changes in morphology after 24 h of treatment. Rutin also induced microglial activation characterized by an increase in OX-42 positive cells and a large proportion of cells with a CD150/CD206-positive M2 phenotype. Rutin also induced a decrease in the mRNA levels of TNF, IL1ß, IL6 and iNOS, reduced the production of IL6, TNF, and nitric oxide, and increased production of the M2 regulatory cytokine IL10 and arginase. Rutin also significantly inhibited the LPS-induced expression of PTGS2, IL18 and TGFß mRNA. These findings show that rutin has the ability to promote microglial proliferation and induces microglial polarization to the M2 profile when cells are stimulated with LPS. These results point this flavonoid as a possible alternative in the treatment or prevention of neurodegenerative disorders.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rutina / Microglia / Receptores de Superfície Celular / Lectinas Tipo C / Lectinas de Ligação a Manose / Membro 1 da Família de Moléculas de Sinalização da Ativação Linfocitária / Anti-Inflamatórios Limite: Animals Idioma: En Revista: Chem Biol Interact Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irlanda
Texto completo
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XML
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Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rutina / Microglia / Receptores de Superfície Celular / Lectinas Tipo C / Lectinas de Ligação a Manose / Membro 1 da Família de Moléculas de Sinalização da Ativação Linfocitária / Anti-Inflamatórios Limite: Animals Idioma: En Revista: Chem Biol Interact Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irlanda